Epitelno mezenhimalna tranzicija u stromi adenokarcinoma in situ i invazivnog adenokarcinoma vrata maternice

master's thesis

• Title (english): Epithelial mesenchymal transition in the stroma of adenocarcinoma in situ and invasive adenocarcinoma of the uterine cervix
• Author: Ivana Omazić
• Mentor: Marina Kos (mentor)
• Committee member: Slavko Orešković (predsjednik povjerenstva)
• Committee member: Slavko Gašparov (član povjerenstva)
• Committee member: Marina Kos (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Pathology); Zagreb
• Defense date and country: 2019-07-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Pathology

Abstract

Često je teško razlikovati adenokarcinom in situ od početne invazije invazivnog adenokarcinoma vrata maternice. Imunohistokemijske promjene povezane s invazijom adenokarcinoma vrata maternice slabo su istražene. Cilj ovog istraživanja bio je utvrditi fenotipske promjene vezane uz epitelno-mezenhimalnu tranziciju u adenokarcinomu in situ i invazivnom adenokarcinomu vrata maternice. Imunohistokemijska ekspresija vimentina, glatkomišićnog aktina, CD34 i E-kadherina određivana je u 10 uzoraka urednog endocervikalnog epitela, 10 uzoraka adenokarcinoma in situ i 10 uzoraka invazivnog adenokarcinoma. Intenzitet imunohistokemijske ekspresije procjenjivan je na skali od 0 do 3 (0=nema ekspresije, 3=jaka ekspresija). U usporedbi s normalnim endocervikalnim epitelom i AIS, pronađeno je pojačano bojanje periglandularne strome IAC na SMA. Reakcija je bila srednje (2) izražena u 6/10 slučajeva IAC, a jaka (3) u samo jednom slučaju IAC. CD34 je bio jako izražen (3) u svim slučajevima normalnog endocervikalnog epitela i AIS. Srednja (2) izraženost CD34 bila je prisutna u 7/10 slučajeva IAC, a niska (1) u 3/10 slučajeva. Intenzitet ekspresije SMA i CD34 ovisio je o dubini invazije atipičnih tumorskih žlijezda. Nisu pronađene razlike u imunohistokemijskoj ekspresiji vimentina i E-kadherina između normalnog endocervikalnog epitela, AIS i IAC. Epitelno-mezenhimalna tranzicija mogla bi biti mehanizam invazije adenokarcinoma vrata maternice. Ipak, potrebna su daljnja istraživanja kako bi se odredilo koji bi se imunohistokemijski markeri mogli koristiti u razlikovanju adenokarcinoma in situ od invazivnog adenokarcinoma vrata maternice.

Abstract (english)

It is often hard to differentiate among adenocarcinoma in situ and early invasive endocervical adenocarcinoma. Little is known of the immunohistochemical changes associated with the invasion of endocervical adenocarcinoma. The aim of this study was to examine the phenotypic changes associated with the epithelial-mesenchymal transition in adenocarcinoma in situ and invasive adenocarcinoma of the uterine cervix. The immunohistochemical expression of vimentin, smooth-muscle actin, CD34 and E-cadherin was assessed in 10 cases of normal endocervical epithelium, 10 cases of adenocarcinoma in situ and 10 cases of invasive adenocarcinoma. The intensity of the immunohistochemical expression was evaluated on a scale from 0 to 3 (0=no expression, 3=high expression). Compared to normal endocervical epithelium and AIS, there was an increase in the staining of the periglandular stroma for SMA in IAC. The expression was moderate (2) in 6/10 cases of IAC and high (3) in only one case of IAC. CD34 was strongly expressed (3) in all cases of normal endocervical epithelium and AIS. Moderate (2) CD34 expression was found in 7/10 cases of IAC and low (1) in 3/10 cases. The intensity of the expression of both SMA and CD34 correlated with the depth of invasion of atypical tumor glands. There was no difference in the immunohistochemical staining for vimentin and E-cadherin among normal endocervical epithelium, AIS and IAC.
Epithelial-mesenchymal transition might be a mechanism of invasion in endocervical adenocarcinoma. However, further studies are required to determine which immunohistochemical markers could be used in the differentiation of adenocarcinoma in situ from invasive adenocarcinoma of the uterine cervix.

• Keywords: adenokarcinom in situ; invazivni adenokarcinom; vrat maternice; epitelno-mezenhimalna tranzicija
• Keywords (english): adenocarcinoma in situ; invasive adenocarcinoma; cervix uteri; epithelial-mesenchymal transition
• Language: croatian
• URN:NBN: urn:nbn:hr:105:143153
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-12-12 10:27:26