prikaz prve stranice dokumenta Comparison of clinical and sewage isolates of Acinetobacter baumannii from two long-term care facilities in Zagreb; mechanisms and routes of spread
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Scientific paper - Original scientific paper
Comparison of clinical and sewage isolates of Acinetobacter baumannii from two long-term care facilities in Zagreb; mechanisms and routes of spread
Archives of Microbiology, 202 (2020), 2; 361-368. https://doi.org/10.1007/s00203-019-01750-9
Bedenić, Branka; Siroglavić, Marko; Slade, Mia; Šijak, Dorotea; Dekić, Svjetlana; Šeruga Musić, Martina; Godan-Hauptman, Ana; Hrenović, Jasna

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Bedenić, B., Siroglavić, M., Slade, M., Šijak, D., Dekić, S., Šeruga Musić, M. ... Hrenović, J. (2020). Comparison of clinical and sewage isolates of Acinetobacter baumannii from two long-term care facilities in Zagreb; mechanisms and routes of spread. Archives of Microbiology, 202. (2), 361-368. doi: 10.1007/s00203-019-01750-9

Bedenić, Branka, et al. "Comparison of clinical and sewage isolates of Acinetobacter baumannii from two long-term care facilities in Zagreb; mechanisms and routes of spread." Archives of Microbiology, vol. 202, no. 2, 2020, pp. 361-368. https://doi.org/10.1007/s00203-019-01750-9

Bedenić, Branka, Marko Siroglavić, Mia Slade, Dorotea Šijak, Svjetlana Dekić, Martina Šeruga Musić, Ana Godan Hauptman and Jasna Hrenović. "Comparison of clinical and sewage isolates of Acinetobacter baumannii from two long-term care facilities in Zagreb; mechanisms and routes of spread." Archives of Microbiology 202, no. 2 (2020): 361-368. https://doi.org/10.1007/s00203-019-01750-9

Bedenić, B., et al. (2020) 'Comparison of clinical and sewage isolates of Acinetobacter baumannii from two long-term care facilities in Zagreb; mechanisms and routes of spread', Archives of Microbiology, 202(2), pp. 361-368. doi: 10.1007/s00203-019-01750-9

Bedenić B, Siroglavić M, Slade M, Šijak D, Dekić S, Šeruga Musić M, and sur.. Comparison of clinical and sewage isolates of Acinetobacter baumannii from two long-term care facilities in Zagreb; mechanisms and routes of spread. Archives of Microbiology [Internet]. 2020 March [cited 2024 April 24];202(2):361-368. doi: 10.1007/s00203-019-01750-9

B. Bedenić, et al., "Comparison of clinical and sewage isolates of Acinetobacter baumannii from two long-term care facilities in Zagreb; mechanisms and routes of spread", Archives of Microbiology, vol. 202, no. 2, pp. 361-368, March 2020. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:692719. [Accessed: 24 April 2024]

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Title (english)Comparison of clinical and sewage isolates of Acinetobacter baumannii from two long-term care facilities in Zagreb; mechanisms and routes of spread
AuthorBranka Bedenić
AuthorMarko Siroglavić
AuthorMia Slade
AuthorDorotea Šijak
AuthorSvjetlana Dekić
AuthorMartina Šeruga Musić
AuthorAna Godan-Hauptman
AuthorJasna Hrenović
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
In the previous studies OXA-23-like and OXA-24-like β-lactamase were reported among Acinetobacter baumannii in both hospitals and long-term care facilities (LTCF) in Croatia. The aim of this study was to analyze clinical and sewage A. baumannii isolates from two nursing homes in Zagreb, with regard to antibiotic susceptibility and resistance mechanisms, to determine the route of spread of carbapenem-resistant isolates. Nine clinical isolates were collected from February to May 2017 whereas in April 2017, ten A. baumannii isolates were collected from sewage of two nursing homes in Zagreb. Antibiotics susceptibility was determined by broth microdilution method. The presence of carbapenemase and extended spectrum β-lactamases (ESBL) encoding genes was explored by PCR. Conjugation and transformation experiments were performed as previously described. Genotyping was performed by SG determination, PFGE and MLST. Seven clinical isolates were positive for blaOXA24-like whereas two clinical and environmental carbapenem-resistant isolates, respectively, were found to possess blaOXA-23-like genes. Attempts to transfer imipenem resistance were unsuccessful indicating chromosomal location of blaOXA-23 gene. All carbapenem-resistant isolates belonged to SG- 1 (IC-2) whereas the rest of the isolates susceptible to carbapenems were allocated to SG- 2 (IC-1). PFGE analysis revealed low degree of genetic variability within both IC- I and IC- II. MLST corroborated that two environmental OXA-23 isolates belong to the ST-195. This study showed dissemination of OXA-23 producing A. baumannii from the nursing home into the urban sewage. Disinfection of nursing home sewage should be recommended in order to prevent the spread of resistance genes into the community sewage.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleArchives of Microbiology
Numberingvol. 202, no. 2, pp. 361-368
p-ISSN0302-8933
e-ISSN1432-072X
DOIhttps://doi.org/10.1007/s00203-019-01750-9
URN:NBNurn:nbn:hr:105:692719
Publication2020-03
Document URLhttp://link.springer.com/10.1007/s00203-019-01750-9
Type of resourceText
Access conditionsOpen access
Embargo expiration date: 2021-04-01
Terms of useLicence In copyright icon
Created on2021-02-23 09:34:39