prikaz prve stranice dokumenta Autologous blood coagulum containing rhBMP6 induces new bone formation to promote anterior lumbar interbody fusion (ALIF) and posterolateral lumbar fusion (PLF) of spine in sheep
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Scientific paper - Original scientific paper
Autologous blood coagulum containing rhBMP6 induces new bone formation to promote anterior lumbar interbody fusion (ALIF) and posterolateral lumbar fusion (PLF) of spine in sheep
Bone, 138 (2020); 115448. https://doi.org/10.1016/j.bone.2020.115448
Grgurević, Lovorka; Erjavec, Igor; Gupta, Munish; Pećin, Marko; Bordukalo-Nikšić, Tatjana; Štoković, Nikola; Vnuk, Dražen; Farkaš, Vladimir; Capak, Hrvoje; Milošević, Milan; Bubić Špoljar, Jadranka; Perić, Mihaela; Vučković, Mirta; Matičić, Dražen; Windhager, Reinhard; Oppermann, Hermann; Sampath, T. Kuber; Vukičević, Slobodan More authors...

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Grgurević, L., Erjavec, I., Gupta, M., Pećin, M., Bordukalo Nikšić, T., Štoković, N. ... Vukičević, S. (2020). Autologous blood coagulum containing rhBMP6 induces new bone formation to promote anterior lumbar interbody fusion (ALIF) and posterolateral lumbar fusion (PLF) of spine in sheep. Bone, 138.. doi: 10.1016/j.bone.2020.115448

Grgurević, Lovorka, et al. "Autologous blood coagulum containing rhBMP6 induces new bone formation to promote anterior lumbar interbody fusion (ALIF) and posterolateral lumbar fusion (PLF) of spine in sheep." Bone, vol. 138, 2020. https://doi.org/10.1016/j.bone.2020.115448

Grgurević, Lovorka, Igor Erjavec, Munish Gupta, Marko Pećin, Tatjana Bordukalo Nikšić, Nikola Štoković, Dražen Vnuk, et al. "Autologous blood coagulum containing rhBMP6 induces new bone formation to promote anterior lumbar interbody fusion (ALIF) and posterolateral lumbar fusion (PLF) of spine in sheep." Bone 138 (2020). https://doi.org/10.1016/j.bone.2020.115448

Grgurević, L., et al. (2020) 'Autologous blood coagulum containing rhBMP6 induces new bone formation to promote anterior lumbar interbody fusion (ALIF) and posterolateral lumbar fusion (PLF) of spine in sheep', Bone, 138. doi: 10.1016/j.bone.2020.115448

Grgurević L, Erjavec I, Gupta M, Pećin M, Bordukalo Nikšić T, Štoković N, and sur.. Autologous blood coagulum containing rhBMP6 induces new bone formation to promote anterior lumbar interbody fusion (ALIF) and posterolateral lumbar fusion (PLF) of spine in sheep. Bone [Internet]. 2020 September [cited 2024 April 26];138. doi: 10.1016/j.bone.2020.115448

L. Grgurević, et al., "Autologous blood coagulum containing rhBMP6 induces new bone formation to promote anterior lumbar interbody fusion (ALIF) and posterolateral lumbar fusion (PLF) of spine in sheep", Bone, vol. 138, September 2020. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:660078. [Accessed: 26 April 2024]

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Title (english)Autologous blood coagulum containing rhBMP6 induces new bone formation to promote anterior lumbar interbody fusion (ALIF) and posterolateral lumbar fusion (PLF) of spine in sheep
AuthorLovorka Grgurević
AuthorIgor Erjavec
AuthorMunish Gupta
AuthorMarko Pećin
AuthorTatjana Bordukalo-Nikšić
AuthorNikola Štoković
AuthorDražen Vnuk
AuthorVladimir Farkaš
AuthorHrvoje Capak
AuthorMilan Milošević
AuthorJadranka Bubić Špoljar
AuthorMihaela Perić
MBZ: 222361
AuthorMirta Vučković
AuthorDražen Matičić
AuthorReinhard Windhager
AuthorHermann Oppermann
AuthorT. Kuber Sampath
AuthorSlobodan Vukičević
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Anatomy and Clinical Anatomy)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Anatomy
Abstract (english)
In the present study, we evaluated an autologous bone graft substitute (ABGS) composed of recombinant human BMP6 (rhBMP6) dispersed within autologous blood coagulum (ABC) used as a physiological carrier for new bone formation in spine fusion sheep models. The application of ABGS included cervical cage for use in the anterior lumbar interbody fusion (ALIF), while for the posterolateral lumbar fusion (PLF) sheep model allograft devitalized bone particles (ALLO) were applied with and without use of instrumentation. In the ALIF model, ABGS (rhBMP6/ABC/cage) implants fused significantly when placed in between the L4-L5 vertebrae as compared to control (ABC/cage) which appears to have a fibrocartilaginous gap, as examined by histology and micro CT analysis at 16 weeks following surgery. In the PLF model, ABGS implants with or without ALLO showed a complete fusion when placed ectopically in the gutter bilaterally between two decorticated L4-L5 transverse processes at a success rate of 88% without instrumentation and at 80% with instrumentation; however the bone volume was 50% lower in the instrumentation group than without, as examined by histology, radiographs, micro CT analyses and biomechanical testing at 27 weeks following surgery. The newly formed bone was uniform within ABGS implants resulting in a biomechanically competent and histologically qualified fusion with an optimum dose in the range of 100 μg rhBMP6 per mL ABC, while in the implants that contained ALLO, the mineralized bone particles were substituted by the newly formed remodeling bone via creeping substitution. These findings demonstrate for the first time that ABGS (rhBMP6/ABC) without and with ALLO particles induced a robust bone formation with a successful fusion in sheep models of ALIF and PLF, and that autologous blood coagulum (ABC) can serve as a preferred physiological native carrier to induce new bone at low doses of rhBMP6 and to achieve a successful spinal fusion.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleBone
Numberingvol. 138, 115448
p-ISSN8756-3282
e-ISSN1873-2763
DOIhttps://doi.org/10.1016/j.bone.2020.115448
URN:NBNurn:nbn:hr:105:660078
Publication2020-09
Document URLhttps://linkinghub.elsevier.com/retrieve/pii/S8756328220302283
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-02-04 09:04:10