Procjena staničnog imunog odgovora QuantiFERON Monitor testom u primatelja bubrega s infektivnim komplikacijama

professional thesis

• Title (english): Evaluation of cell-mediated immune response by QuantiFERON Monitor Assay in kidney transplant recipients presenting with infective complications
• Author: Ivan Margeta
• Mentor: Ivana Mareković (mentor)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2024-07-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Internal Medicine

Abstract

Teško je procijeniti ukupnu razinu imunosupresije kod bolesnika s transplantiranim bubregom. QuantiFERON Monitor (QFM) je in vitro dijagnostički test koji mjeri razinu otpuštanja interferona-γ (IFN-γ) u perifernoj krvi. Cilj naše studije bilo je usporediti rezultate
QFM testa kod stabilnih bolesnika s transplantiranim bubregom i kod bolesnika koji imaju akutno infektivno zbivanje. Istraživanje je provedeno u našem Centru te je uključen 71 bolesnik. Podijeljeni su u dvije skupine ovisno o kliničkoj slici (stabilni bolesnici ili bolesnici s akutnom infekcijom). Nije bilo statistički značajnih razlika u razini otpuštanja IFN-γ između dvije skupine bolesnika (stabilni bolesnici 140.59 ± 215.28 IU/ml, bolesnici s akutnom infekcijom 78.37 ± 197.03 IU/ml, P = .24). Dodatnom analizom nađena je statistički značajna razlika u otpuštanju interferona kod bolesnika s bakterijskim infekcijama u usporedbi sa stabilnim bolesnicima (26.52 ± 42.46 IU/ml vs 140.59 ± 215.28 IU/ml, P = .04). QFM test može biti koristan alat za pomoć prilikom vođenja imunosupresivne terapije kod bolesnika s transplantiranim bubregom, ali potrebne su daljnje studije kako bi se potvrdila njegova
dijagnostička vrijednost.

Abstract (english)

The net level of immunosuppression in kidney transplant recipients is difficult to assess. QuantiFERON Monitor (QFM) is an in vitro diagnostic test that detects interferon-γ (IFN-γ) release in peripheral blood. The aim of our study was to compare QFM testing results in stable kidney transplant recipients and kidney transplant recipients with infection, in a single-centre cohort.We enrolled 71 kidney transplant recipients from our transplantation centre. They were divided into 2 groups according to clinical presentation (Stable kidney transplant recipients or Infection).There were no significant differences in interferon-γ release between the 2 groups (Stable kidney transplant recipients 140.59 ± 215.28 IU/ml, Infection group 78.37 ± 197.03 IU/ml, P = .24). A further analysis revealed that kidney transplant recipients presenting with bacterial infection had significantly lower IFN-γ release when compared to stable kidney transplant recipients (26.52 ± 42.46 IU/ml vs 140.59 ± 215.28 IU/ml, P = .04). Kidney transplant recipients presenting with bacterial infection had lower IFN-γ release when compared to stable kidney transplant recipients. The QFM test may be useful as a tool to help guide immunosuppression dosing in kidney transplant recipients, but further studies are required to confirm its diagnostic value.

• Keywords: imunosupresija; infekcija; transplantacija bubrega; QuantiFERON Monitor
• Keywords (english): immunosuppression; infection; kidney transplantation; QuantiFERON Monitor
• Language: croatian
• URN:NBN: urn:nbn:hr:105:520681
• Study programme: Title: Nephrology; Study programme type: university; Study level: postgraduate specialist; Academic / professional title: sveučilišni/a magistar/magistra nefrologije (sveučilišni/a magistar/magistra nefrologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-08-21 10:40:44