Sastav bakterijske mikrobiote darovanog humanog mlijeka

doctoral thesis

• Title (english): Composition of the bacterial microbiota of donated human milk
• Author: Jurjana Novoselac
• Mentor: Branka Golubić Ćepulić (mentor)
• Mentor: Zrinka Bošnjak (komentor); MBZ: 319631
• Committee member: Arjana Tambić Andrašević (predsjednik povjerenstva)
• Committee member: Duška Tješić-Drinković (član povjerenstva)
• Committee member: Sanja Pleština (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Gynecology and Obstetrics); Zagreb
• Defense date and country: 2025-01-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Medical Microbiology
• Universal decimal classification (UDC): 618 - Gynaecology. Obstetrics

Abstract

Uvod: Istraživanja su pokazala utjecaj različitih obilježja na sastav bakterijske mikrobiote humanog mlijeka (HMM), no nisu provođena na uzorcima iz mješavina darovanog humanog mlijeka (DHM).
Cilj: Cilj istraživanja bio je identificirati sastav bakterijske mikrobiote DHM-a te istražiti postoje li obilježja koja mogu utjecati na zastupljenost rodova pojedinih bakterija.
Ispitanice i metode: Iz arhivskih uzoraka mješavina DHM-a izolirana je bakterijska deoksiribonukleinska kiselina. Sekvenciranjem nove generacije određena je zastupljenost operativnih taksonomskih jedinica bakterija i uspoređena s poznatim obilježjima darivateljica, njihove djece i darovanog mlijeka.
Rezultati: U istraživanje je uključeno 88 uzoraka DHM-a od 88 darivateljica. U zastupljenosti bakterijskih rodova između skupina uzoraka podijeljenih prema načinu poroda i izloženosti antibioticima nije bilo značajnih razlika. Razlike su nađene s obzirom na tjedne gestacije na porodu i tjedne laktacije, način hranjenja majčinim mlijekom, način izdajanja viška mlijeka, broj bakterija kultiviranih u mlijeku prije pasterizacije te duljinu trajanja i temperaturu pohrane.
Zaključak: Dojenje djece te kraća pohrana na -30 ⁰C u Banci ovim su se istraživanjem pokazali kao pozitivni prediktori za zastupljenost rodova Lactobacillus i Bifidobacetrium kao dio bakterijske HMM-e u uzorcima mlijeka.

Abstract (english)

Introduction: Studies have shown the influence of different characteristics on the composition of the bacterial microbiota of human milk (HMM), they were not conducted on samples from pools of donated human milk (DHM).
Aim: The aim of the research was to identify the composition of the bacterial microbiota of DHM and to investigate whether there are characteristics that can influence the representation of certain bacterial genera.
Subjects and methods: Bacterial deoxyribonucleic acid was isolated from archival samples from pools of DHM. Using next generation sequencing, the representation of operational taxonomic units of bacteria was determined and compared with the known characteristics of donors, their children and DHM.
Results: 88 DHM samples were included in the research. There were no significant differences in the representation of bacterial genera between sample groups divided according to the mode of delivery and exposure to antibiotics. Differences were found regarding weeks of gestation at delivery and weeks of lactation, the method of feeding with breast milk, the method of expressing surplus milk, the number of bacterial colonies cultivated in milk before pasteurization, and the length of time and temperature of storage.
Conclusion: Breastfeeding of children and shorter storage at -30 ⁰C in the human milk bank have been shown as positive predictors for the presence of the genus Lactobacillus and Bifidobactrium as part of bacterial HMM in milk samples.

• Keywords: bakterija; mikrobiota; humano mlijeko; sekvenciranje nove generacije; dojenje; Lactobacillus; Bifidobacterium
• Keywords (english): bacteria; microbiota; human milk; next generation sequencing; breastfeeding; Lactobacillus; Bifidobacterium
• Language: croatian
• URN:NBN: urn:nbn:hr:105:282851
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
• Type of resource: Text
• Extent: 117 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-01-13 07:57:22