Unique miRNA Expression Profile in MSI- and EMAST-Unstable Sporadic Colon Cancer

Scientific paper - Original scientific paper

• Author: Sonja Marinović
• Author: Kristina Vuković Đerfi
• Author: Anita Škrtić
• Author: Mirko Poljak
• Author: Sanja Kapitanović
• Author's institution: University of Zagreb; School of Medicine; (Department of Pathology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Pathology

Abstract (english)

MicroRNAs (miRNAs) are critical post-transcriptional gene regulators and their involvement in sporadic colon cancer (CRC) tumorigenesis has been confirmed. In this study we investigated differences in miRNA expression in microsatellite stable (MSS/EMAST-S), microsatellite unstable marked by high elevated microsatellite alterations at selected tetranucleotide repeats (MSS/EMAST-H), and high microsatellite unstable (MSI-H/EMAST-H) tumor subgroups as well as in tumors with different clinicopathologic characteristics. An RT-qPCR analysis of miRNA expression was carried out on 45 colon cancer and adjacent normal tissue samples (15 of each group). Overall, we found three differentially expressed miRNAs between the subgroups. miR-92a-3p and miR-224-5p were significantly downregulated in MSI-H/EMAST-H tumors in comparison to other subgroups. miR-518c-3p was significantly upregulated in MSS/EMAST-H tumors in comparison to stable and highly unstable tumors. Furthermore, we showed that miR-143-3p and miR-145-5p were downregulated in tumors in comparison to normal tissues in all subgroups. In addition, we showed overexpression of miR-125b-5p in well-differentiated tumors and miR-451a in less advanced tumors. This is the first report on differences in miRNA expression profiles between MSS/EMAST-S, MSS/EMAST-H, and MSI-H/EMAST-H colorectal cancers. Our findings indicate that the miRNA expression signatures differ in CRC subgroups based on their instability status.

• Keywords (english): Aged; Colonic Neoplasms / genetics; Colonic Neoplasms / pathology; Female; Gene Expression Profiling / methods; Gene Expression Regulation, Neoplastic; Humans; Male; MicroRNAs / genetics; Microsatellite Instability; Microsatellite Repeats / genetics; Middle Aged; Transcriptome / genetics
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: Genes
• Numbering: vol. 15, no. 8, 1007
• e-ISSN: 2073-4425
• DOI: https://doi.org/10.3390/genes15081007
• URN:NBN: urn:nbn:hr:105:743821
• Publication: 2024-08-01
• Project: Number: HRZZ-IP-2016-06-1430; Title (croatian): Mikrosatelitna nestabilnost (MSI i EMAST) u molekularnom profiliranju sporadičnih karcinoma debelog crijeva; Title (english): Microsatellite instability (MSI and EMAST) in molecular profiling of sporadic colon cancers; Acronym: MSIandEMASTinCRC; Jurisdiction: Croatia; Funding stream: Research Projects
• Document URL: https://www.mdpi.com/2073-4425/15/8/1007
• Type of resource: Text
• Access conditions: Open access
• Created on: 2025-03-25 11:43:19