MicroRNA-193a-3p as a Valuable Biomarker for Discriminating between Colorectal Cancer and Colorectal Adenoma Patients

Scientific paper - Original scientific paper

• Author: Marija Fabijanec
• Author: Andrea Hulina-Tomašković
• Author: Mario Štefanović
• Author: Donatella Verbanac
• Author: Ivana Ćelap
• Author: Anita Somborac-Bačura
• Author: Marija Grdić Rajković
• Author: Alma Demirović
• Author: Snježana Ramić
• Author: Božo Krušlin
• Author: Lada Rumora
• Author: Andrea Čeri
• Author: Martha Koržinek
• Author: József Petrik
• Author: Neven Ljubičić
• Author: Neven Baršić
• Author: Karmela Barišić
• Author's institution: University of Zagreb; School of Medicine; (Department of Internal Medicine)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Internal Medicine

Abstract (english)

Specific markers for colorectal cancer (CRC), preceded by colorectal adenoma (pre-CRC), are lacking. This study aimed to investigate whether microRNAs (miR-19a-3p, miR-92a-3p, miR-193a-3p, and miR-210-3p) from tissues and exosomes are potential CRC biomarkers and compare them to existing biomarkers, namely carcinoembryonic antigen (CEA) and carbohydrate antigen (CA) 19-9. MiRNA was isolated in the samples of 52 CRC and 76 pre-CRC patients. Expression levels were analyzed by RT-qPCR. When comparing pre-CRC and CRC tissue expression levels, only miR-193a-3p showed statistically significant result (p < 0.0001). When comparing the tissues and exosomes of CRC samples, a statistically significant difference was found for miR-193a-3p (p < 0.0001), miR-19a-3p (p < 0.0001), miR-92a-3p (p = 0.0212), and miR-210-3p (p < 0.0001). A receiver-operating characteristic (ROC) curve and area under the ROC curve (AUC) were used to evaluate the diagnostic value of CEA, CA 19-9, and miRNAs. CEA and CA 19-9 had good diagnostic values (AUCs of 0.798 and 0.668). The diagnostic value only of miR-193a-3p was highlighted (AUC = 0.725). The final logistic regression model, in which we put a combination of CEA concentration and the miR-193a-3p expression level in tissues, showed that using these two markers can distinguish CRC and pre-CRC in 71.3% of cases (AUC = 0.823). MiR-193a-3p from tissues could be a potential CRC biomarker.

• Keywords (english): Adenoma / diagnosis; Adenoma / genetics; Adenoma / metabolism; Adult; Aged; Aged, 80 and over; Biomarkers, Tumor / genetics; CA-19-9 Antigen; Carcinoembryonic Antigen / genetics; Carcinoembryonic Antigen / metabolism; Colorectal Neoplasms / diagnosis; Colorectal Neoplasms / genetics; Colorectal Neoplasms / metabolism; Diagnosis, Differential; Exosomes / genetics; Exosomes / metabolism; Female; Gene Expression Regulation, Neoplastic; Humans; Male; MicroRNAs / genetics; Middle Aged; ROC Curve
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: International Journal of Molecular Sciences
• Numbering: vol. 25, no. 15, 8156
• e-ISSN: 1422-0067
• DOI: https://doi.org/10.3390/ijms25158156
• URN:NBN: urn:nbn:hr:105:713889
• Publication: 2024-07-26
• Project: Number: IP-2019-04-4624; Title (croatian): Gensko, proteinsko i RNA profiliranje kolorektalnog karcinoma primjenom tekuće biopsije; Title (english): Genetic, Protein and RNA Profiling of Colorectal Cancer Using Liquid Biopsy; Acronym: CRCMolProfil; Leader: Karmela Barišić; Jurisdiction: Croatia; Funding stream: Research Projects
• Project: Number: KK.01.1.1.02.0021; Title (croatian): Jačanje znanstveno-istraživačkih i inovacijskih kapaciteta Farmaceutsko-biokemijskog fakulteta Sveučilišta u Zagrebu; Title (english): -; Acronym: FarmInova; Leader: Jasmina Lovrić; Jurisdiction: eu; Funding stream: European Regional Development Fund
• Document URL: https://www.mdpi.com/1422-0067/25/15/8156
• Type of resource: Text
• Access conditions: Open access
• Created on: 2025-03-31 07:45:16