Utjecaj ishemije na in vitro diferencijaciju živčanih matičnih stanica izoliranih iz mišjeg soja THY1 YFP16

master's thesis

• Title (english): Influence of ischemia on in vitro differentiation of neural stem cells isolated from mouse strain THY1 YFP16
• Author: Barbara Šimunić
• Mentor: Dinko Mitrečić (mentor)
• Committee member: Marina Radmilović (predsjednik povjerenstva)
• Committee member: Željka Krsnik (član povjerenstva)
• Committee member: Dinko Mitrečić (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Histology and Embryology); Zagreb
• Defense date and country: 2017-09-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Cytology, Histology and Embryology

Abstract

Ishemija mozga dovodi do teških posljedica na moždano tkivo, a moždani udar je jedan od najvećih problema moderne medicine. Budući da matične stanice imaju veliki potencijal u liječenju raznih bolesti, njihova trasplantacija je predložena kao moguća metoda u smanjivanju štetnih posljedica ishemije na moždano tkivo. Ipak, vrlo se malo zna o utjecaju ishemije na same matične stanice. Cilj ovog istraživanja je bio utvrditi utjecaj ishemije na ponašanje i diferencijaciju živčanih matičnih stanica uzgajanih u kulturi. Nakon izolacije matičnih stanica iz mišjih zametaka, napravljena je analiza kretanja stanica, njihovog rasta i diferencijacije uz pomoć visokorezolucijskog mikroskopa EVOS te njegovog poluautomatskog softvera Cell Tracker. Nakon što su stanice izložene ishemiji, analiziran je niz parametara (ukupna prijeđena udaljenost svih stanica, maksimalna dosegnuta brzina, maksimalna prijeđena udaljenost po jednoj stanici i udio naglih skretanja po stanici), a statistički značajna razlika je nađena u ukupnoj udaljenosti koje pređu sve stanice u kulturi. Ovim istraživanjem smo uspostavili nov način analize utjecaja različitih čimbenika na matične stanice, te smo pokazali da ishemija mijenja njihovo ponašanje.

Abstract (english)

Brain ischemia leads to serious brain damage and stroke is one of the major problems of modern medicine. Since stem cells have great potential in treating various diseases, their transplantation is suggested as a possible method of reducing the damaging consequences of ischemia on brain tissue. Still, it is not much known about the effect of ischemia on the stem cells. The aim of this study was to determine the effect of ischemia on the behavior and differentiation of neural stem cells grown in the culture. After isolation of stem cells from mouse embryos, analysis of cell movement, their growth and differentiation was made with the help of a high-resolution EVOS microscope and its semi-automatic Cell Tracker software. After the cells were exposed to ischemia, a number of parameters were analyzed (the length of the total traveled distance of all cells, the maximum speed, the maximum distance reached per cell and the rate of sudden rotation per cell). The statistically significant difference was found in the length of the total traveled distance of all cells. This research has established a new way of analyzing the effects of various factors on stem cells, and we have shown that ischemia changes their behavior.

• Keywords: matične stanice; ishemija; živčani sustav; in vitro mikroskopija uživo
• Keywords (english): stem cells; ischemia; nervous system; in vitro live microscopy
• Language: croatian
• URN:NBN: urn:nbn:hr:105:622183
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-10-30 13:10:15