Usporedba zastupljenosti uzročnika uretritisa detektiranih u mokraći pacijenata šireg područja grada Zagreba

master's thesis

• Title (english): Comparison of the representation of urethritis-causing pathogens in the urine of patients from the wider metropolitan area of Zagreb
• Author: Jakov Jurčević
• Mentor: Jasmina Vraneš (mentor)
• Committee member: Sunčanica Ljubin Sternak (predsjednik povjerenstva)
• Committee member: Goran Tešović (član povjerenstva)
• Committee member: Jasmina Vraneš (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Medical Microbiology and Parasitology); Zagreb
• Defense date and country: 2018-07-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Medical Microbiology

Abstract

Cilj istraživanja bila je detekcija spolno-prenosivih (SP) patogena u sedimentu prvog mlaza urina uzetog umjesto obriska uretre u 822 muškaraca. Uzorci su obojani i kultivirani te upotrijebljeni u multipleks PCR testu. Uretritis je utvrđen u svega 72/822 (8,76%)
pacijenata detekcijom signifikantnog broja polimorfonuklearnih leukocita u uzorcima, a etiološka dijagnoza postavljena u njih 68 od 72. Najčešći uzročnik uretritisa bila je Chlamydia trachomatis (47,22%), a zatim Mycoplasma genitalium (12,50%) i Neisseria
gonorrhoeae (9,72%). U pacijenata s negonokoknim uretritisom dominantni uzročnik bila je C. trachomatis, dok je M. genitalium utvrđena kao drugi uzročnik negonokoknog uretritisa. Mikrobiološka analiza je najčešće tražena zbog probira ili kontrole (340/41,36%) te u pacijenata s kroničnim prostatitisom (105/12,77%). SP patogeni detektirani su značajno češće u pacijenata s uretritisom nego u pacijenata iz ostalih dijagnostičkih skupina (p<0,01), ali nije bilo statistički značajne razlike u detekciji U. urealyticum između dijagnostičkih skupina (p>0,05).

Abstract (english)

The aim of study was detection of sexually transmitted (ST) pathogens in sediment of first-void urine of 822 men, collected instead of urethral swab. Samples were stained, cultivated and used in multiplex PCR test. Urethritis was confirmed in only 72/822 (8,76%) patients by detecting a significant number of polymorphonuclear leukocytes in the samples, and etiologic
diagnosis was established in 68/72 men with urethritis. The most common cause of urethritis was Chlamydia trachomatis (47,22%), followed by Mycoplasma genitalium (12,50%) and Neisseria gonorrhoeae (9,72%). In patients with nongonococcal urethritis dominant cause was C. trachomatis, whereas M. genitalium was found as second most common NGU pathogen. Microbiological analysis was required the most frequently for screening and control purposes (340/41,36%) and in patients with chronic prostatitis (105/12,77%). ST pathogens were detected more frequently in patients with urethritis than in patients from other diagnostic groups (p<0.01), while U. urealyticum was detected without statistically significant difference among
diagnostic groups (p>0.05).

• Keywords: uretritis; molekularna dijagnostika; multipleks PCR
• Keywords (english): urethritis; molecular diagnostics; multiplex PC
• Language: croatian
• URN:NBN: urn:nbn:hr:105:657391
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-01-16 12:15:17