Izraženost proteina TCF1 u difuznim karcinomima želuca

master's thesis

• Title (english): TCF1 protein expression in diffuse gastric carcinoma
• Author: Nina Fajs
• Mentor: Tamara Nikuševa Martić (mentor)
• Committee member: Frane Paić (predsjednik povjerenstva)
• Committee member: Ljiljana Šerman (član povjerenstva)
• Committee member: Tamara Nikuševa Martić (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Medical Biology); Zagreb
• Defense date and country: 2019-07-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Clinical Cytology
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Internal Medicine

Abstract

Wnt signalni put je uključen u patogenezu brojnih karcinoma, a u nekih je dokazana i pojačana aktivnost nizvodnih transkripcijskih faktora, kao što su proteini TCF/LEF obitelji. Jedna od ovih neoplazmi je i karcinom želuca, koji se histološki prema Laurénovoj klasifikaciji može podijeliti na tri tipa: intestinalni, difuzni i miješani. Karcinom želuca je pri vrhu ljestvice smrtnosti od karcinoma u svijetu, te se brojnim istraživanjima žele otkriti nove terapijske mogućnosti koje bi poboljšale preživljenje i kvalitetu života oboljelih. Cilj ovog istraživanja bilo je dokazati da postoji razlika u ekspresiji TCF1 proteina u normalnom tkivu i tkivu difuznog karcinoma želuca, s pretpostavkom da bi ovaj protein mogao imati ulogu u patogenezi ove vrste karcinoma. Analizirano je 36 uzoraka karcinoma te 43 uzoraka normalnog tkiva prikupljenih iz arhive Kliničkog zavoda za patologiju i citologiju KBC-a Zagreb. Nakon primjene imunohistokemijskog bojanja, napravljena je stereološka kvantitativna analiza te je otkriven značajna razlika (p< 0,05) u ekspresiji TCF1 proteina između stanica u zdravom tkivu i malignih stanica difuznog karcinoma želuca. Ovim rezultatom smo zaključili da bi čimbenik Wnt signalnog puta, protein TCF1, mogao imati ulogu u patogenezi difuznih karcinoma želuca, te su potrebne daljnja istraživanja o ulozi ovog proteina i mogućnosti da se ova spoznaja primjeni u terapijskom postupku.

Abstract (english)

Wnt signal pathway is involved in the pathogenesis of multiple cancer types. Moreover, upregulation of downstream transcription factors, such as TCF/LEF family, is proved in several of them. One of this neoplasms is stomach cancer, which is, according to Laurén classification histologically divided into three categories: intestinal, diffuse and mixed type. Stomach cancer is nearly at the top of the list of leading causes of death from malignant diseases worldwide, and many studies search for new treatment possibilities which would improve survival and quality of life of patients suffering from this disease. The aim of this study was to prove that there is a difference in expression of TCF1 protein between normal tissue and diffuse stomach cancer, with the assumption that this protein may have a role in the pathogenesis of this type of stomach cancer. 36 samples of diffuse gastric carcinoma and 43 samples of normal stomach tissue gathered at the Clinical Institute of Pathology and Cytology of the Zagreb University Hospital Centre were analyzed. After the use of immunohistochemical staining, stereological quantitative analysis was done and a statistically significant difference (p< 0,05) in expression of TCF1 protein between healthy tissue cells and the cells of diffuse gastric carcinoma was found. With this result we concluded that this part of the Wnt signaling pathway could have a role in the pathogenesis of diffuse gastric carcinoma. Further studies are needed to explore the role of TCF1 protein and its potential use in new therapic procedures.

• Keywords: difuzni karcinom želuca; Wnt signalni put; transkripcijski faktori; TCF1; genske promjene
• Keywords (english): diffuse gastric carcinoma; Wnt signaling pathway; transcription factors; TCF1; genetic abnormalities
• Language: croatian
• URN:NBN: urn:nbn:hr:105:857021
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-02-19 11:37:56