prikaz prve stranice dokumenta Circulating miRNA Expression Profiling in Primary Aldosteronism
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Scientific paper - Original scientific paper
Circulating miRNA Expression Profiling in Primary Aldosteronism
Frontiers in Endocrinology, 10 (2019); 739. https://doi.org/10.3389/fendo.2019.00739
Decmann, Abel; Nyírö, Gábor; Darvasi, Ottó; Turai, Péter; Bancos, Irina; Kaur, Ravinder Jeet; Pezzani, Raffaele; Iacobone, Maurizio; Kraljević, Ivana; Kaštelan, Darko; Parasiliti-Caprino, Mirko; Maccario, Mauro; Nirschl, Nina; Heinrich, Daniel; Reincke, Martin; Patócs, Attila; Igaz, Peter More authors...

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Decmann, A., Nyírö, G., Darvasi, O., Turai, P., Bancos, I., Kaur, R. J. ... Igaz, P. (2019). Circulating miRNA Expression Profiling in Primary Aldosteronism. Frontiers in Endocrinology, 10.. doi: 10.3389/fendo.2019.00739

Decmann, Abel, et al. "Circulating miRNA Expression Profiling in Primary Aldosteronism." Frontiers in Endocrinology, vol. 10, 2019. https://doi.org/10.3389/fendo.2019.00739

Decmann, Abel, Gábor Nyírö, Ottó Darvasi, Péter Turai, Irina Bancos, Ravinder Jeet Kaur, Raffaele Pezzani, et al. "Circulating miRNA Expression Profiling in Primary Aldosteronism." Frontiers in Endocrinology 10 (2019). https://doi.org/10.3389/fendo.2019.00739

Decmann, A., et al. (2019) 'Circulating miRNA Expression Profiling in Primary Aldosteronism', Frontiers in Endocrinology, 10. doi: 10.3389/fendo.2019.00739

Decmann A, Nyírö G, Darvasi O, Turai P, Bancos I, Kaur RJ, and sur.. Circulating miRNA Expression Profiling in Primary Aldosteronism. Frontiers in Endocrinology [Internet]. 2019 October 29 [cited 2024 October 05];10. doi: 10.3389/fendo.2019.00739

A. Decmann, et al., "Circulating miRNA Expression Profiling in Primary Aldosteronism", Frontiers in Endocrinology, vol. 10, October 2019. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:319946. [Accessed: 05 October 2024]

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Metadata
Title (english)Circulating miRNA Expression Profiling in Primary Aldosteronism
AuthorAbel Decmann
AuthorGábor Nyírö
AuthorOttó Darvasi
AuthorPéter Turai
AuthorIrina Bancos
AuthorRavinder Jeet Kaur
AuthorRaffaele Pezzani
AuthorMaurizio Iacobone
AuthorIvana Kraljević
AuthorDarko Kaštelan
AuthorMirko Parasiliti-Caprino
AuthorMauro Maccario
AuthorNina Nirschl
AuthorDaniel Heinrich
AuthorMartin Reincke
AuthorAttila Patócs
AuthorPeter Igaz
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Internal Medicine)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Internal Medicine
Abstract (english)
Objective: Primary aldosteronism is a major cause of secondary hypertension. Its two principal forms are bilateral adrenal hyperplasia (BAH) and aldosterone-producing adenoma (APA) whose differentiation is clinically pivotal. There is a major clinical need for a reliable and easily accessible diagnostic biomarker for case identification and subtyping. Circulating microRNAs were shown to be useful as minimally invasive diagnostic markers. Our aim was to determine and compare the circulating microRNA expression profiles of adenoma and hyperplasia plasma samples, and to evaluate their applicability as minimally invasive markers. ----- Methods: One hundred and twenty-three samples from primary aldosteronism patients were included. Next-generation sequencing was performed on 30 EDTA-anticoagulated plasma samples (discovery cohort). Significantly differently expressed miRNAs were validated by real-time reverse transcription-qPCR in an independent validation cohort (93 samples). ----- Results: We have found relative overexpression of miR-30e-5p, miR-30d-5p, miR-223-3p, and miR-7-5p in hyperplasia compared to adenoma by next-generation sequencing. Validation by qRT-PCR confirmed significant overexpression of hsa-miR-30e-5p, hsa-miR-30d-5p, and hsa-miR-7-5p in hyperplasia samples. Regarding the microRNA expressional variations, adenoma is more heterogeneous at the miRNA level compared to hyperplasia. ----- Conclusion: Three microRNAs were significantly overexpressed in hyperplasia samples compared to adenoma samples, but their sensitivity and specificity values are not good enough for introduction to clinical practice.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleFrontiers in Endocrinology
Numberingvol. 10, 739
e-ISSN1664-2392
DOIhttps://doi.org/10.3389/fendo.2019.00739
URN:NBNurn:nbn:hr:105:319946
Publication2019-10-29
Document URLhttps://www.frontiersin.org/article/10.3389/fendo.2019.00739/full
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2021-02-23 08:43:11