B regulatory cells and monocyte subpopulations in patients with chronic graft-vs-host disease

Scientific paper - Original scientific paper

• Author: Antonija Babić
• Author: Lejla Kurić
• Author: Ana Zelić Kerep
• Author: Lana Desnica
• Author: Antonela Lelas
• Author: Milan Milošević
• Author: Ranka Serventi-Seiwerth
• Author: Nadira Duraković
• Author: Zinaida Perić
• Author: Marinka Mravak Stipetić
• Author: Ervina Bilić
• Author: Romana Čeović
• Author: Marko Barešić
• Author: Tamara Vukić
• Author: Dina Ljubas Kelečić
• Author: Sanja Mazić
• Author: Ines Bojanić
• Author: Ana Hećimović
• Author: Ernest Bilić
• Author: Renata Zadro
• Author: Radovan Vrhovac
• Author: Steven Z. Pavletić
• Author: Drago Batinić
• Author: Dražen Pulanić
• Author's institution: University of Zagreb; School of Medicine; (Department of Dermatology)
• Author's institution: University of Zagreb; School of Medicine; (Department of Physiology and Immunology)
• Author's institution: University of Zagreb; School of Medicine; (Department of Internal Medicine)
• Author's institution: University of Zagreb; School of Medicine; (Department of Neurology)
• Author's institution: University of Zagreb; School of Medicine; (Department of Pediatrics)
• Author's institution: University of Zagreb; School of Medicine; (Department of Environmental and Occupational Health)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Internal Medicine
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Neurology
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Human Physiology
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Public Health and Health Care; Occupational and Sports Medicine
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Dermatovenerology
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Pediatrics

Abstract (english)

Aim: To assess the correlations of B regulatory cells (Bregs) and monocyte subsets in peripheral blood with the National Institutes of Health (NIH)-consensus-defined clinical manifestations of chronic graft-vs-host disease (cGvHD), in an attempt to establish their role as cellular biomarkers.
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Methods: This multidisciplinary prospective study enrolled adult cGVHD patients treated in the University Hospital Center Zagreb and University of Zagreb School of Medicine. Immunophenotypic subpopulations of CD24highCD38high Bregs (CD27-, CD27+, and total) and monocyte (classical, intermediate, and non-classical) counts were correlated with demographic, transplant, and cGVHD-related data. Bivariate correlation analysis was performed to evaluate the correlations between Bregs and monocytes subsets and cGVHD organ involvement, as well as cGVHD severity and immunosuppression intensity.
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Results: Twenty-two adult patients (54.5% female) with cGVHD were enrolled. The median (range) age was 44.5 years (24-65). All patients were transplanted for hematologic malignancies and 40.9% had severe NIH cGVHD global score. The median time from cGVHD diagnosis to the analysis was 16.6 months (0-176). The organ most frequently affected with cGVHD were the eyes (68.2%), skin (45.5%), lungs (45.5%), and liver (40.9%). Lower total and CD27-Bregs counts were correlated with worse cGVHD severity, higher immunosuppression intensity, and lung cGVHD, in terms of cell count, but also with skin cGVHD, in terms of percentages. Patients with liver and joint/fascia cGVHD had a lower percentage of non-classical monocytes and patients with more severe global NIH score had a higher classical monocytes count.
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Conclusion: Different organs affected by cGVHD are differently associated with different subpopulations of Bregs and monocytes.

• Keywords (english): Adult; Aged; Chronic Disease; Female; Graft vs Host Disease; Hematopoietic Stem Cell Transplantation; Humans; Male; Middle Aged; Monocytes; Prospective Studies; United States; Young Adult
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: Croatian Medical Journal
• Numbering: vol. 62, no. 2, pp. 154-164
• p-ISSN: 0353-9504
• e-ISSN: 1332-8166
• DOI: https://doi.org/10.3325/cmj.2021.62.154
• URN:NBN: urn:nbn:hr:105:284963
• Publication: 2021-04-30
• Document URL: http://www.cmj.hr/2021/62/2/33938655.htm
• Type of resource: Text
• Access conditions: Open access
• Created on: 2021-05-06 08:01:51