Heterogeneity of murine periosteum progenitors involved in fracture healing

Scientific paper - Original scientific paper

• Author: Brya G Matthews
• Author: Sanja Novak
• Author: Francesca V Sbrana
• Author: Jessica L Funnell
• Author: Ye Cao
• Author: Emma J Buckels
• Author: Danka Grčević
• Author: Ivo Kalajzić
• Author's institution: University of Zagreb; School of Medicine; (Croatian Institiute for Brain Research)
• Author's institution: University of Zagreb; School of Medicine; (Department of Physiology and Immunology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Human Physiology
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Neuroscience

Abstract (english)

The periosteum is the major source of cells involved in fracture healing. We sought to characterize progenitor cells and their contribution to bone fracture healing. The periosteum is highly enriched with progenitor cells, including Sca1+ cells, fibroblast colony-forming units, and label-retaining cells compared to the endosteum and bone marrow. Using lineage tracing, we demonstrate that alpha smooth muscle actin (αSMA) identifies long-term, slow-cycling, self-renewing osteochondroprogenitors in the adult periosteum that are functionally important for bone formation during fracture healing. In addition, Col2.3CreER-labeled osteoblast cells contribute around 10% of osteoblasts but no chondrocytes in fracture calluses. Most periosteal osteochondroprogenitors following fracture can be targeted by αSMACreER. Previously identified skeletal stem cell populations were common in periosteum but contained high proportions of mature osteoblasts. We have demonstrated that the periosteum is highly enriched with skeletal progenitor cells, and there is heterogeneity in the populations of cells that contribute to mature lineages during periosteal fracture healing.

• Keywords (english): alpha smooth muscle actin; fracture; mouse; osteoblast; pdgfra; periosteum; regenerative medicine; skeletal stem cell; stem cells
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: eLife
• Numbering: vol. 10, e58534
• e-ISSN: 2050-084X
• DOI: https://doi.org/10.7554/eLife.58534
• URN:NBN: urn:nbn:hr:105:189659
• Publication: 2021-02-09
• Document URL: https://elifesciences.org/articles/58534
• Type of resource: Text
• Access conditions: Open access
• Created on: 2021-09-22 12:39:42