prikaz prve stranice dokumenta Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion
  Download
PDF 2.01 MB
Scientific paper - Original scientific paper
Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion
International Journal of Molecular Sciences, 22 (2021), 20; 10989. https://doi.org/10.3390/ijms222010989
Majali-Martinez, Alejandro; Weiss-Fuchs, Ursula; Miedl, Heidi; Forstner, Desiree; Bandres-Meriz, Julia; Hoch, Denise; Djelmis, Josip; Ivanišević, Marina; Hiden, Ursula; Gauster, Martin; Desoye, Gernot

Cite this document

Majali Martinez, A., Weiss Fuchs, U., Miedl, H., Forstner, D., Bandres Meriz, J., Hoch, D. ... Desoye, G. (2021). Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion. International Journal of Molecular Sciences, 22. (20). doi: 10.3390/ijms222010989

Majali Martinez, Alejandro, et al. "Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion." International Journal of Molecular Sciences, vol. 22, no. 20, 2021. https://doi.org/10.3390/ijms222010989

Majali Martinez, Alejandro, Ursula Weiss Fuchs, Heidi Miedl, Desiree Forstner, Julia Bandres Meriz, Denise Hoch, Josip Djelmis, et al. "Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion." International Journal of Molecular Sciences 22, no. 20 (2021). https://doi.org/10.3390/ijms222010989

Majali Martinez, A., et al. (2021) 'Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion', International Journal of Molecular Sciences, 22(20). doi: 10.3390/ijms222010989

Majali Martinez A, Weiss Fuchs U, Miedl H, Forstner D, Bandres Meriz J, Hoch D, and sur.. Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion. International Journal of Molecular Sciences [Internet]. 2021 October 12 [cited 2024 November 29];22(20). doi: 10.3390/ijms222010989

A. Majali Martinez, et al., "Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion", International Journal of Molecular Sciences, vol. 22, no. 20, October 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:979714. [Accessed: 29 November 2024]

Please login to the repository to save this object to your list.
Metadata
Title (english)Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion
AuthorAlejandro Majali-Martinez
AuthorUrsula Weiss-Fuchs
AuthorHeidi Miedl
AuthorDesiree Forstner
AuthorJulia Bandres-Meriz
AuthorDenise Hoch
AuthorJosip Djelmis
AuthorMarina Ivanišević
AuthorUrsula Hiden
AuthorMartin Gauster
AuthorGernot Desoye
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Gynecology and Obstetrics)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Gynecology and Obstetrics
Abstract (english)
Type 1 diabetes mellitus (T1DM) is associated with reduced fetal growth in early pregnancy, but a contributing role of the placenta has remained elusive. Thus, we investigated whether T1DM alters placental development in the first trimester. Using a protein array, the level of 60 cell-cycle-related proteins was determined in human first trimester placental tissue (gestational week 5–11) from control (n = 11) and T1DM pregnancies (n = 12). Primary trophoblasts (gestational week 7–12, n = 32) were incubated in the absence (control) or presence of hyperglycemia (25 mM D-glucose) and hyperosmolarity (5.5 mM D-glucose + 19.5 mM D-mannitol). We quantified the number of viable and dead trophoblasts (CASY Counter) and assessed cell cycle distribution (FACS) and trophoblast invasion using a transwell assay. T1DM was associated with a significant (p < 0.05) downregulation of Ki67 (−26%), chk1 (−25%), and p73 (−26%). The number of viable trophoblasts was reduced under hyperglycemia (−23%) and hyperosmolarity (−18%), whereas trophoblast invasion was increased only under hyperglycemia (+6%). Trophoblast cell death and cell cycle distribution remained unaffected. Collectively, our data demonstrate that hyperglycemia decreases trophoblast proliferation as a potential contributing factor to the reduced placental growth in T1DM in vivo.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 22, no. 20, 10989
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms222010989
URN:NBNurn:nbn:hr:105:979714
Publication2021-10-12
Document URLhttps://www.mdpi.com/1422-0067/22/20/10989
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-04-01 10:03:43