Povezanost životnih i okolišnih faktora, kvalitete sjemena i N-glikana sjemene tekućine u muškaraca

doctoral thesis

• Title (english): Association of life habits and environmental factors, sperm quality and seminal fluid N-glycans in men
• Author: Tihana Marić
• Mentor: Ana Katušić Bojanac (mentor)
• Mentor: Aleksandra Fučić (komentor)
• Committee member: Floriana Jakuš (predsjednik povjerenstva)
• Committee member: Davor Ježek (član povjerenstva)
• Committee member: Tamara Nikuševa Martić (član povjerenstva)
• Committee member: Tvrtko Hudolin (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2022-04-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Urology
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Medical Biochemistry
• Universal decimal classification (UDC): 612 - Physiology

Abstract

Muška neplodnost je izrazito heterogen poremećaj te je nerijetko osnovna analiza sjemena nedostatna za njezinu dijagnostiku i objašnjenje. Cilj istraživanja je bio po prvi put odrediti povezanost kvalitete spermija i životnih i okolišnih čimbenika sa sastavom N-glikana ukupnih proteina sjemene plazme. U studiju je uključeno 166 ispitanika s normalnim (N = 82) i patološkim (PAT = 84) parametrima sjemena (koncentracija/broj, pokretljivost, vijabilnost, morfologija spermija tj. astenozoospermija, oligozoospermija, oligoastenozoospermija). Fragmentacija DNA spermija (SDF) bila je statistički značajno veća kod skupine PAT te negativno povezana s pokretljivošću i vitalnošću spermija. N-glikan SPGP14 se pokazao statistički najznačajnijim za razlikovanje skupine N i PAT, kao i za statistički značajan trend opadanja u skupini PAT od astenozoospermije prema oligozoospermiji do oligoastenozoospermije. Parametri sjemena i N-glikana nisu pokazali značajne povezanosti, osim kod srednje progresivne pokretljivosti spermija i SPGP2 u skupini PAT. Specifični N-glikani su pokazali statistički značajnu povezanost s SDF i sažimanja kromatina. Okolišni čimbenici (prehrana, pušenje, alkohol, urbana/ruralna sredina) pokazali su značajnu povezanost sa SDF, sažimanjem kromatina i promjenom sastava N-glikana čija konačna struktura je također istražena. Ovaj doktorski rad daje doprinos sveobuhvatnijem uvidu u mušku neplodnost, a N-glikani iskazuju potencijal kao biomarkeri za dijagnostiku muške neplodnosti.

Abstract (english)

Male infertility is a highly heterogeneous disorder and often basic semen analysis is insufficient for its diagnosis and explanation. The aim of the study was to determine for the first time the relationship between sperm quality and life and environmental factors with the seminal plasma total protein N-glycans. The study included 166 subjects with normal (N = 82) and pathological (PAT = 84) semen parameters (concentration/number, motility, viability, sperm morphology i.e. asthenozoospermia, oligozoospermia, oligoastenozoospermia). Sperm DNA fragmentation (SDF) was statistically significantly higher in the PAT group and negatively associated with sperm motility and vitality. The N-glycan SPGP14 was statistically the most significant for distinguishing the N and PAT group, and for a statistically significant decreasing trend in the PAT group from asthenozoospermia to oligozoospermia and oligoastenozoospermia. Semen parameters and N-glycans showed no significant association, except for medium progressive sperm motility and SPGP2 in the PAT group. Specific N-glycans showed a statistically significant association with SDF and chromatin condensation. Environmental factors (diet, smoking, alcohol, urban/rural living) showed a significant association with SDF, chromatin condensation and changes in N-glycans which final structures were also investigated. This doctoral thesis contributes to a more comprehensive insight into male infertility, and N-glycans express potential as biomarkers in male infertility diagnostics.

• Keywords: Sjeme; Fragmentacija DNK; Pokretljivost spermija; Astenozoospermija; Muška neplodnost; Sperma; Biomarkeri; Polisaharidi
• Keywords (english): Seed; DNA fragmentation; Sperm motility; Asthenozoospermia; Male infertility; Sperm; Biomarkers; Polysaccharides
• Language: croatian
• URN:NBN: urn:nbn:hr:105:042326
• Project: Number: KK.01.1.1.01.0008; Title: Znanstveni centar izvrsnosti za reproduktivnu i regenerativnu medicinu – istraživanja novih platformi i potencijala; Acronym: CERRM; Jurisdiction: eu; Funder: EK - Europska komisija
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
• Type of resource: Text
• Extent: 113 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-04-25 10:06:55