Uloga FTIR spektroskopije u ranoj dijagnostici glaukoma

master's thesis

• Title (english): The role of FTIR spectroscopy in early diagnostics of glaucoma
• Author: Lorena Karla Rudež
• Mentor: Marko Škrabić (mentor)
• Committee member: Ozren Gamulin (predsjednik povjerenstva)
• Committee member: Sanja Dolanski Babić (član povjerenstva)
• Committee member: Marko Škrabić (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Physics and Biophysics); Zagreb
• Defense date and country: 2021-07-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences

Abstract

Glaukom je jedan od najčešćih uzroka ireverzibilne sljepoće u svijetu. Najbolji ishodi liječenja mogući su samo uz što raniju dijagnozu ove bolesti. Trenutno ne postoje identificirani glaukom-specifični biomarkeri niti adekvatne metode probira glaukoma u populaciji. Stoga smo odlučili analizirati strukturu očnih vodica koristeći FTIR spektroskopiju koja nudi jedinstvenu priliku za istraživanje sastava tvari na molekularnoj razini. U studiji je sudjelovalo 87 ispitanika, podijeljenih u dvije skupine: 1) 55 pacijenata s glaukomom (158 uzoraka očne vodice) i 2) kontrolna grupa od 32 pacijenta s kataraktom (93 uzorka očne vodice). Svi uzorci očne vodice prikupljeni su na početku operacije glaukoma ili katarakte. Uzorci su posušeni na prozirnim silicijskim pločicama te su njihovi spektri snimljeni FTIR spektroskopijom i potom analizirani metodama statističke analize: STT, PCA i SVM. Naše je istraživanje pokazalo da FTIR spektroskopija razlikuje strukturu medija očne vodice pacijenata s glaukomom od pacijenata s kataraktom. Izgrađen je i model za prepoznavanje spektara koji se odnose na odgovarajuću patologiju. Rezultati FTIR spektroskopije analizirani statističkim metodama STT, PCA i SVM pokazali se kao vrlo obećavajuća metoda za uspješno razlikovanje uzoraka očne vodice pacijenata oboljelih od glaukoma i pacijenata oboljelih od katarakte, stoga ova metoda predstavlja osnovu za razvoj nove metode rane dijagnostike glaukoma.

Abstract (english)

Glaucoma is one of the major causes of irreversible blindness in the world. Detecting glaucoma as early as possible ensures the best possible treatment and outcome. Since there are currently no glaucoma-specific biomarkers identified and no glaucoma screening method available, we decided to analyze molecular structure of aqueous humor using FTIR spectroscopy which offers a unique opportunity to investigate the composition of substances on a molecular basis. The study included 87 age matched subjects, divided into two groups 1) 55 glaucoma patients (158 aqueous humor samples) and 2) a control group comprising 32 patients with cataracts (93 aqueous humor samples). All aqueous humor samples were collected at the start of the glaucoma or cataract surgery. FTIR spectra, of the samples dried on transparent silicon plates, were recorded and analyzed by statistical methods of STT, PCA and SVM. Our study has shown that FTIR spectroscopy can differentiate media structure between the control group and the glaucoma patient group. A model for spectra recognition attributable to a certain type of pathology was established. Results of FTIR spectroscopy analyzed by statistical methods of STT, PCA and SVM have proven to be a promising method for differentiation of aqueous humor samples between patients with cataract and glaucoma, therefore this method can form the basis for the development of new methods in early diagnostics of glaucoma.

• Keywords: glaukom; FTIR; očna vodica
• Keywords (english): glaucoma; FTIR; aqueous humor
• Language: croatian
• URN:NBN: urn:nbn:hr:105:909352
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-05-05 12:41:43