Novel Therapeutic Effects in Rat Spinal Cord Injuries: Recovery of the Definitive and Early Spinal Cord Injury by the Administration of Pentadecapeptide BPC 157 Therapy

Scientific paper - Original scientific paper

• Author: Darko Perović
• Author: Marija Milavić
• Author: Stjepan Dokuzović
• Author: Ivan Krezić
• Author: Slaven Gojković
• Author: Hrvoje Vranes
• Author: Igor Bebek
• Author: Vide Bilić
• Author: Nenad Somun
• Author: Ivan Brižić
• Author: Ivan Skorak
• Author: Klaudija Hriberski
• Author: Sunčana Sikirić
• Author: Eva Lovrić
• Author: Sanja Štrbe; MBZ: 319335
• Author: Milovan Kubat
• Author: Alenka Boban Blagaić
• Author: Anita Škrtić
• Author: Sven Seiwerth
• Author: Predrag Sikirić
• Author's institution: University of Zagreb; School of Medicine; (Department of Pharmacology)
• Author's institution: University of Zagreb; School of Medicine; (Department of Pathology)
• Author's institution: University of Zagreb; School of Medicine; (Department of Forensic Medicine)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Forensic Medicine
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Pathology
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Pharmacology

Abstract (english)

Recently, marked therapeutic effects pertaining to the recovery of injured rat spinal cords (1 min compression injury of the sacrocaudal spinal cord (S2-Co1) resulting in tail paralysis) appeared after a single intraperitoneal administration of the stable gastric pentadecapeptide BPC 157 at 10 min post-injury. Besides the demonstrated rapid and sustained recovery (1 year), we showed the particular points of the immediate effect of the BPC 157 therapy that began rapidly after its administration, (i) soon after injury (10 min), or (ii) later (4 days), in the rats with a definitive spinal cord injury. Specifically, in counteracting spinal cord hematoma and swelling, (i) in rats that had undergone acute spinal cord injury, followed by intraperitoneal BPC 157 application at 10 min, we focused on the first 10–30 min post-injury period (assessment of gross, microscopic, and gene expression changes). Taking day 4 post-injury as the definitive injury, (ii) we focused on the immediate effects after the BPC 157 intragastric application over 20 min of the post-therapy period. Comparable long-time recovery was noted in treated rats which had definitive tail paralysis: (iii) the therapy was continuously given per orally in drinking water, beginning at day 4 after injury and lasting one month after injury. BPC 157 rats presented only discrete edema and minimal hemorrhage and increased Nos1, Nos2, and Nos3 values (30 min post-injury, (i)) or only mild hemorrhage, and only discrete vacuolation of tissue (day 4, (ii)). In the day 4–30 post-injury study (iii), BPC 157 rats rapidly presented tail function recovery, and no demyelination process (Luxol fast blue staining).

• Keywords (english): pentadecapeptide BPC 157; recovery; spinal cord definitive injury; spinal cord early injury; therapy
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: Current Issues in Molecular Biology
• Numbering: vol. 44, no. 5, pp. 1901-1927
• e-ISSN: 1467-3045
• DOI: https://doi.org/10.3390/cimb44050130
• URN:NBN: urn:nbn:hr:105:066238
• Publication: 2022-04-27
• Document URL: https://www.mdpi.com/1467-3045/44/5/130
• Type of resource: Text
• Access conditions: Open access
• Created on: 2022-12-07 15:07:46