Transplantacija hondrocita u liječenju hrskavice

master's thesis

• Title (english): Chondrocyte transplantation in cartilage regeneration
• Author: Karlo Videc
• Mentor: Mislav Jelić (mentor)
• Committee member: Domagoj Delimar (predsjednik povjerenstva)
• Committee member: Tomislav Đapić (član povjerenstva)
• Committee member: Mislav Jelić (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Orthopedics); Zagreb
• Defense date and country: 2022-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Orthopedics

Abstract

Hrskavica prekriva površine kostiju u zglobu i ima ključnu ulogu u funkciji zgloba. Tvore ju izvanstanični matriks i stanice koje se nazivaju hondrociti. Oštećenja hrskavice imaju vrlo malu sposobnost cijeljenja i zbog toga mogu dovesti do značajnog morbiditeta. Iako su vrlo česta, sve do kraja prošlog stoljeća njihovo liječenje nije pružalo dobre dugoročne rezultate. Metode koje potiču regeneraciju hrskavice su stoga vrlo atraktivne, budući da mlađim pacijentima omogućuju povratak prijašnjim aktivnostima i sprječavaju razvoj osteoartritisa. Metoda autologne transplantacije hondrocita danas ima važnu ulogu u liječenju žarišnih oštećenja hrskavice visokog stupnja. U ovoj metodi, mali uzorak zdrave hrskavice se artroskopijom uzima iz područja pod manjim opterećenjem i šalje u laboratorij na obradu. Izolirani hondrociti se umnožavaju u staničnoj kulturi. Nakon što se dobije dovoljna količina stanica, pacijentu se u drugom zahvatu autologni hondrociti implantiraju u područje oštećenja. Prednost je korištenje pacijentovih vlastitih stanica, što dovodi do smanjenog rizika za odbacivanje transplantata, dok su nedostaci dugotrajna rehabilitacija i visoka cijena, zbog čega su zahtjevi za njezinom učinkovitosti mnogo veći nego kod korištenja jednostavnijih zahvata, poput mikrofraktura. Temeljem novih spoznaja, tijekom vremena razvijene su različite modifikacije ove metode, s ciljem smanjenja komplikacija i povećanja uspješnosti zahvata. Mnoga istraživanja potvrdila su uspješnost i dugoročne rezultate autologne transplantacije hondrocita.

Abstract (english)

Articular cartilage covers the bone surfaces in the joint and plays a key role in joint function. It is made up of an extracellular matrix and cells called chondrocytes. Cartilage damage has very limited capacity of healing and can therefore lead to significant morbidity. Although very common, until the end of the last century their treatment did not provide good long-term results. Methods that stimulate cartilage regeneration are therefore very attractive, as they allow younger patients to return to previous activities and prevent the development of osteoarthritis. The method of autologous chondrocyte transplantation today plays an important role in the treatment of focal high-grade chondral defects. In this method, a small sample of healthy cartilage is taken from the area under less load by arthroscopy and sent to the laboratory for processing. The isolated chondrocytes are expanded in cell culture. After a suffcient amount of cells is obtained, autologous chondrocytes are implanted into the defect in the second procedure. The advantage is the use of the patient's own cells, which reduces the risk of transplant rejection, while the disadvantages are long rehabilitation duration and high cost, which makes the requirements for its effectiveness much higher than when using simpler procedures, such as microfracture. Based on new findings, various modifications of this method have been developed over time, with the aim of reducing complications and increasing the success of the procedure. Many studies have confirmed the success and long-term results of autologous chondrocyte transplantation.

• Keywords: transplantacija hondrocita; hrskavica
• Keywords (english): chondrocyte transplantation; cartilage
• Language: croatian
• URN:NBN: urn:nbn:hr:105:584692
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-03-20 09:52:39