Prognostička vrijednost intratumorske heterogenosti ekspresije beta-katenina i E-kadherina u planocelularnim karcinomima grkljana

doctoral thesis

• Title (english): Prognostic value of intratumoral heterogeneity of beta-catenin and e-kadherin in the squamous cell laryngeal carcinoma
• Author: Ankica Vasilj; MBZ: 224925
• Mentor: Sven Seiwerth (mentor)
• Committee member: nije dostupno (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2011-07-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences
• Universal decimal classification (UDC): 61 - Medical sciences

Abstract

U studiju je uključeno 90 bolesnika operiranih u Klinici za bolesti uha, nosa i grla, KBC Zagreb u periodu od 2000.-2005. godine. Imunohistokemijskim protutijelima na E-kadherin i beta-katenin (DAKO) obojani su dobiveni preparati. Određen je TNM, gradus i lokalizacija tumora, a imunohistokemijska reakcija je procjenjena semikvantitativno na membrani i u citoplazmi u tri područja tumora; transformacijskom rubu, centru i invazivnom rubu. Ekspresija membranskog i citoplazmatskog E-kadherina, te citoplazmatskog i membranskog beta-katenina značajno se razlikovala unutar tumorskih regija. Invazivni rub pokazao je znatno slabiju ekspresiju ovog membranskog kompleksa. Jaka ekspresija citoplazmatskog beta-katenina u centru tumora češća je kod slabo diferenciranih karcinoma grkljana. Rekurzivnim particioniranjem veličina tumora je izdvojena kao jedini signifikantan prognostički parametar, pri čemu tumori veličine T1, T2 i T3 imaju bolje preživljenje u odnosu na tumore veličine T4. Tumori veličine T1, T2 i T3 ukoliko imaju visoku razinu ekspresije membranskog E-kadherina u centru tumora, imaju tri puta manji rizik od umiranja. S druge strane, tumori veličine T4 imaju četiri puta manji rizik od umiranja ukoliko su dobro diferencirani odnosno gradusa G1. Tumori veličine T4, umjereno i slabo diferencirani odnosno gradusa G2 i G3 imaju šest puta manji rizik umiranja ukoliko imaju visoku ekspresiju membranskog beta-katenina u transformacijskom rubu.

Abstract (english)

The study included 90 patients with laryngeal squamous cell carcinoma. Immunohistochemical reaction with anti E-kadherin and beta-catenin antibodies was accessed using a scoring system (0-3), estimating membranic and cytoplasmatic immunopositivity at three separate locations; transformational zone, tumor center and invasive margin of the tumor. A significant difference of expression between tumor regions was found for both membranic and cytoplasmatic E-kadherin and beta-catenin. The invasive segment showed the poorest membranic reaction. Correlation was found beetwen the poorest expression for both membranic E-kadherin and beta-catenin in the invasive margin and good histological differentiation of the tumor. RPART statistical program revealed tumor size as an single statistically significant prognostic parameter, in the way that tumors sizes T1,T2 and T3 have better survival than tumors size T4. Tumor sizes T1,T2 and T3 with high expression of membranic E-kadherin in the tumor center have three times lower risk of death. On the other hand, tumors size T4 have four times lower risk of death if they are well differentiated i.e. gradus G1. Tumor sizes T4, moderate and poor differentiated (gradus 2 i 3) have six times lower risk of death if they have high expression of membranic beta-catenin in the transformational zone of the tumor.

• Keywords: medicina
• Keywords (english): medicine
• Language: croatian
• URN:NBN: urn:nbn:hr:105:941618
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstva (doktor/doktorica znanosti, područje biomedicine i zdravstva)
• Type of resource: Text
• Extent: 100 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-05-05 06:38:03