Učinak pentadekapeptida BPC 157 na produljeni QT interval u štakora uzrokovan ponavljanim dozama antipsihotika i antiemetika

doctoral thesis

• Title (english): The influence of pentadecapeptide BPC 157 on prolonged QT interval in rats caused with repetitive doses of antipsyhotics and antiemetics
• Author: Dean Strinić; MBZ: 351050
• Mentor: Predrag Sikirić (mentor)
• Committee member: nije dostupno (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2015-10-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences
• Universal decimal classification (UDC): 61 - Medical sciences

Abstract

Cilj istraživanja je bio praćenje nastanka srčanih aritmija u štakora davanjem
repetitivnih visokih doza neuroleptika/antiemetika, utvrditi mogućnost njihovog
liječenja primjenom BPC 157 i uvidjeti da li je njegov terapijski učinak ovisan o
primjenjenoj dozi. U istraživanju smo u 150 Wistar štakora davali BPC 157 u
dozama od 10 mcg/kg i 10 ng/kg eksperimentalnoj skupini ili FO 5ml/kg
kontrolnoj skupini intraperitonealno, i to odmah nakon primjene
neuroleptika/antiemetika. Terapijski učinak BPC 157 promatrali smo kroz sat
vremena. Elektrokardiogram smo pratili prije, pola sata i sat nakon primjene
neuroleptika/antiemetika i BPC 157/fiziološke otopine te smo isti postupak
ponavljali svakodnevno kroz 7 dana uz određivanje frekvencije srca, PQ, QRS i
QT intervala. Dobijeni rezultati su ukazali da su štakori koji su dobijali
neuroleptik/antiemetik i BPC 157 imali znatno kraći QT interval nego životinje iz
kontrolne skupine (p<0,05). Dokazali smo da primjenjeni neuroleptici/antiemetici
značajno produljuju QT interval, dok domperidon nema takav učinak, jer nakon
njegove primjene duljina QT intervala ostaje gotovo nepromijenjena. Nadalje,
dokazali smo da navedeni patološki učinak na QT interval, koji su izazvali
neuroleptici i antiemetici neutraliziramo primjenom pentadekapepetida BPC 157
u dozama 10 mcg/kg i 10 ng/kg.
Ovo istraživanje je trebalo rasvijetliti mogućnost djelovanja BPC 157 na
prolongirani QT interval uzrokovan repetitivnim uzimanjem neuroleptika i
antiemetika, jer do sada nemamo adekvatan terapijski odgovor prolongiranom
QT intervalu.

Abstract (english)

Repetitive use of different neuroleptics and antiemetics can lead to prolonged
QT interval. The aims of our research were to investigate whether the use of
pentadecapeptide BPC 157 can prevent the occurence of prolonged QT interval
and is this effect dose related. We used 150 Wistar rats in investigation and
after the application of neuroleptics/antiemetics, in experimental group
intraperitoneally was applied BPC 157 in doses of 10 mcg/kg and 10 ng/kg and
in control group saline in dose of 5 mg/kg. The therapeutic effect of the BPC
157 was followed-up during one hour. The electrocardiogram was determined
before and 30 minutes and one hour after the application of
neuroleptics/antiemetics and BPC 157/saline with assessment of the heart rate,
PQ, QRS and QT intervals. The same procedure was repeated every day
during seven days. We have proven that applied neuroleptics/antiemetics
significantly prolong QT interval, and domperidone has no such effect, because
after its aplication, QT intervalremains unchanged. Furthemore, we have shown
that the patological effect on the QT interval caused by neuroleptics and
antiemetics, can be neutralize by using BPC 157at doses of 10 mg/kg or 10
ng/kg.
This research should demonstrate new possibilities in using BPC 157 after
repetitive doses of neuroleptics/antiemetics because there is still no adequate
therapy for prolonged QT interval.

• Keywords: medicina
• Keywords (english): medicine
• Language: croatian
• URN:NBN: urn:nbn:hr:105:640844
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstva (doktor/doktorica znanosti, područje biomedicine i zdravstva)
• Type of resource: Text
• Extent: 101 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-05-05 07:42:57