Učinak pentadekapeptida BPC 157 na plućno srce u štakora inducirano monokrotalinom

doctoral thesis

• Title (english): Effect of pentadecapeptide BPC 157 on monocrotaline induced cor pulmonale in rat
• Author: Mario Udovičić
• Mentor: Sven Seiwerth (mentor)
• Mentor: Predrag Sikirić (komentor)
• Committee member: nije dostupno (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2016-02-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences
• Universal decimal classification (UDC): 61 - Medical sciences

Abstract

Pentadekapeptid BPC 157 modulira sintezu NO, ima antiaritmijski učinak i protektivno
djelovanje na endotel. U ovoj studiji cilj je bio ispitati njegov učinak u štakorskom
modelu plućnog srca induciranog monokrotalinom. Ova studija je uključila 13 grupa po
6 životinja (mužjaci, tjelesne mase 150-200 g) .
Pentadekapeptid BPC 157 je davan svakodnevno, u vodi za piće ili per os ad libitum u
dvjema dozama (10 μg/kg ili 10 ng/kg), u kotretmanu od 1. do 29. dana ili pak u
posttretmanu, u kojem se za početak tretmana se čekao 15. dan i uspostava plućne
hipertenzije, te su ga životinje primale od 15. dana do 29. dana.
U kontrolnim skupinama dokazana je izrazita hipertrofija desne klijetke, a histološki je
bilo vidljivo masivno zadebljanje medije prekapilarnih arterija, uz kliničko pogoršanje
zbog plućne hipertenzije tijekom 4. tjedna nakon injekcije MCT te smrtnošću među
pokusnim životinjama uslijed desnostranog srčanog zatajivanja. U kotretmanu
pentadekapeptid BPC 157 je u svim modalitetima primjene učinkovito spriječio
nastanak plućne hipertenzije sa svim manifestacijama, dok je u posttretmanu
pentadekapeptid BPC 157 isto tako u svim modalitetima primjene uspješno sanirao
već razvijenu PAH. Zaključujemo da pentadekapeptid BPC 157 uspješno prevenira i
terapira MCT induciranu plućnu hipertenziju i plućno srce u štakora.

Abstract (english)

Pentadecapeptide BPC 157 modulates sythesis of NO, has antiarrhythmic effect and
protective effect on endothelium. This study was designed to investigate its effects in
monocrotaline induced pulmonary hypertension in rats. It included 13 groups by 6 rats
(body weight 150-200 g). Pentadecapeptide BPC 157 was given daily, either in
drinking water or intraperitoneally, in two different dosages (10 μg/kg i 10 ng/kg), in
cotreatment protocol day 1 - 29, while in posttreatment protocol therapeutic
intervention with pentadecapeptide BPC 157 was started when the development of
pulmonary hypertension had already commenced and was upheld day 15 - 29. In
control groups a marked right heart hypertrophy was evident, and massive thickening
of the precapillary artery smooth muscle layer was histologically apparent, with cilincal
deterioration due to pulmonary hypertension during the 4. week after MCT injection,
and some animals dying during this period because of right heart failure. In cotreatment
protocol pentadecapeptide BPC 157 consistently prevented in all application
modalities the development of pulmonary hypertension with all its manifestations, while
in posttreatment therapeutic protocol BPC 157 reversed also in all application
modalities the already established PAH. We conclude that pentadecapeptide BPC 157
prevents and attenuates MCT-induced pulmonary hypertension and cor pulmonale in
rats.

• Keywords: medicina
• Keywords (english): medicine
• Language: croatian
• URN:NBN: urn:nbn:hr:105:362090
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstva (doktor/doktorica znanosti, područje biomedicine i zdravstva)
• Type of resource: Text
• Extent: 112 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-05-05 07:46:35