Mastociti u fetalnim sjemenicima čovjeka

doctoral thesis

• Title (english): Mast cells of the human foetal testis
• Author: Trpimir Goluža
• Mentor: Davor Ježek (mentor)
• Committee member: nije dostupno (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2016-06-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences
• Universal decimal classification (UDC): 61 - Medical sciences

Abstract

O fetalnim mastocitima ljudskog sjemenika u dostupnoj literaturi praktički nema
podataka. Hipoteza ove disertacije je da broj mastocita postupno raste s gestacijskom dobi
fetusa te da se razvoj ovih stanica podudara spovećanjemvolumena cjelokupnog sjemenika i
njegovih struktura. Stoga je cilj ovog rada bio prikazati mastocite u parenhimu fetalnog
sjemenika odgovarajućim histološkim tehnikama te istražiti razvojnu dinamiku ovih stanica
tijekom intrauterinog razvoja.U radu je korišten fetalni materijal dobiven uobičajenom
pedopatološkom sekcijom 39 spontano pobačenih plodova u dobi od 15. do 36. tjedna
gestacije. Tijekom analize korištene su kvalitativne i kvantitativne histološke metode. U
ranijim fazama razvoja (15.-29. tjedan) mastociti su smješteni isključivo unutar tunike
albugineje fetalnog sjemenika. Tek od 30. tjedna gestacije pa sve do kraja ispitivanog
razdoblja (36. tjedan) mastocitinaseljavaju i rahlo vezivo tunike vaskuloze te vezivne
pregrade/rahlo vezivo u dubini parenhima fetalne muške spolne žlijezde. Ukupan broj
mastocita postupno raste tijekom ispitivanog razdoblja razvoja. Rezultati kvantitativne
histološke analize ukazuju na podudarnost u razvojnoj dinamici mastocita ipovećanja
volumena sjemenika,spolnih tračaka i intersticija. Za razliku od toga promjer spolnih tračaka
se bitno ne mijenja. Pretpostavlja se da je razvoj mastocita u fetalnom ljudskom sjemeniku
uvjetovan različitim parakrinim čimbenicima stanica koje izgrađuju intersticij i spolne tračke.
Ovi čimbenici, ukoliko se izoliraju, mogli bi u budućnosti biti primjenjeni u terapiji
neplodnosti muškarca, osobito u slučajevima neopstruktivne azoospermije.

Abstract (english)

In the literature there are no available data about mast cells in the human fetal testis.
The hypothesis of this study was that the number of mast cells increases with gestational age
of the foetus, coinciding with the increase of volumes, sex cords and interstitium. The aim
was to visualize mast cells within the parenchyma of the foetal testis by histological staining
methods and investigate the development of these cells during the intrauterine development of
the whole testis and its structures. The material was obtained during the routine
paedopathological autopsy of 39 spontaneously aborted/stillborn foetuses between 15 and 36
gestational weeks. Qualitative and quantitative analysis was done. During gestational weeks
15-29, mast cells were exclusively located within the tunica albuginea. After 30th gestational
week mast cells, in addition to tunica albuginea, mast cells populated the loose connective
tissue of the tunica vasculosa and the connective tissue of septa/interstitium within the
parenchyma. The total number of mast cells increased over the investigated period.
Quantitative sterological analysis showed that the development of mast cells coincided with
the increase of the volumes of the testis, sex cords and the interstitium. In contrast to these
results, diameter of the sex cords remains similar. The development of mast cells in the foetal
testis is probably regulated by paracrine factors secreted by cells of the interstitium and sex
cords. Once isolated, they could present possible targets for treatment of the male infertility.

• Keywords: medicina
• Keywords (english): medicine
• Language: croatian
• URN:NBN: urn:nbn:hr:105:441890
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstva (doktor/doktorica znanosti, područje biomedicine i zdravstva)
• Type of resource: Text
• Extent: 111 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-05-05 08:09:21