Uloga polimorfizma veznoga mjesta Sp1 gen COL1A1 u ranjivosti ligamenata i tetiva

doctoral thesis

• Author: Bojan Dojčinović
• Mentor: Saša Janković (mentor)
• Mentor: Rajko Kušec (komentor)
• Committee member: nije dostupno (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2016-12-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences
• Universal decimal classification (UDC): 61 - Medical sciences

Abstract

Više do sada objavljenih radova implicira genetsku predispoziciju za ozljedu tetiva i
ligamenata. Istraživanja su potvrdila ovu pretpostavku za ozljedu ligamenata, ali ne i za tetive.
Prilikom rekonstrukcije puknute prednje križne sveze koriste se vlastite tetive kao presadak
koji zamjenjuje ligament. Istraživanje je provedeno na pacijentima operiranim u Zavodu za
ortopediju KB Sveti Duh u razdoblju od lipnja 2007. do lipnja 2012. godine. Retrospektivno
su iz operacijskih protokola izdvojeni pacijenti koji su operirani zbog rerupture prednje križne
sveze, odnosno rupture grafta tetiva semitendinosusa i gracilisa. Od operiranih pacijenata
izdvojili smo one koji su u postoperativnom periodu odnosno nakon završene rehabilitacije
imali rupture tetivnih graftova. Iz uzorka su isključeni pacijenti kod kojih je došlo do rupture
tetivnog grafta u razdoblju godinu dana od operacije, a čemu je uzrok najvjerojatnije vezan uz
samu operaciju ili neadekvatno provedenu rehabilitaciju. Kontrolnu skupinu činili su operirani
pacijenti koji nisu imali ponovnu ozljedu, a po unaprijed definiranim parametrima bili su što
sličniji ispitanicima. Provedena je DNA analiza na polimorfizam Sp1 veznog mjesta COL1A1
gena te su dobiveni rezultati ukazali na povećanu sklonost za ozljeđivanje tetiva u skupini
ispitanika kod kojih se pojavljuje dušična baza timin u genotipu. Također, statističkom je
analizom mlađa dob ispitanika bila faktor koji povećava sklonost ozljeđivanju.

Abstract (english)

Recently published papers implicate a genetic predisposition towards tendon and
ligament injury. Studies have shown that COL1A1 Sp1 binding site polymorphism was
implicated in ACL ruptures, however findings for tendon ruptures are inconclusive. ACL
reconstruction is performed using autologous grafting of gracilis and semitendinosus tendons.
This study was conducted on patients operated by a single surgeon in University Hospital
„Sveti Duh” from June 2007 until June 2012. A retrospective analysis of the operated patients
was performed and a group of patients with graft ruptures was identified. We excluded from
the sample those patients that suffered a reeinjury in the early postoperative period of up to
one year after the surgery. This was done in order to exclude the possible influence of
technical errors during surgery and rehabilitation protocol errors on graft injury. The control
group of patients consisted of injured patents at least one year after surgery that returned to
their preoperative level of activity. The genetic polymorphism of the Sp1 binding site of the
COL1A1 gene was assessed in the DNA extracted from peripheral leukocytes of the two
groups. After statistically interpreting the results of the DNA analysis our conclusion is that
the presence of Thimine (T) on Sp1 binding site of COL1A1 gene increases the risk for
tendon injury in patients operated for ACL rupture. Furthermore, the statistical analysis
performed also identified younger age as a risk factor for tendon injury.

• Keywords: medicina
• Keywords (english): medicine
• Language: croatian
• URN:NBN: urn:nbn:hr:105:680136
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstva (doktor/doktorica znanosti, područje biomedicine i zdravstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-05-05 08:10:16