Učinak pentadekapeptida BPC 157 na posljedice kauterizacije episkleralnih vena štakora

doctoral thesis

• Title (english): The effect of pentadecapeptide BPC 157 on consequences of episcleral vein cauterization in rats
• Author: Tamara Kralj
• Mentor: Predrag Sikirić (mentor)
• Committee member: nije dostupno (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2019-04-03, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences
• Universal decimal classification (UDC): 61 - Medical sciences

Abstract

Ženke Wistar štakora, 220 – 250 g, raspoređene su na tretirane i kontrolne skupine. Model glaukoma u štakora izazvan je kauterizacijom episkleralnih vena: dvije dorzalne vene locirane u blizini m. rectus superiora i jednu inferiornu episkleralnu venu u blizini m. rectus lateralisa. Neposredno prije zahvata prva skupina tretiranih životinja, te nakon toga svakodnevno, primila je BPC 157 (10 μg/kg ili 10 ng/kg), intraperitonealno ili lokalno u obliku kapi 2x2 dnevno. Druga skupina životinja 24 h nakon zahvata primila je pentadekapeptid BPC 157 (10 μg/kg), te nakon toga svakodnevno intraperitonealno ili lokalno u obliku kapi 2x2 dnevno. Kontrolne životinje su istovremeno intraperitonealno ili lokalno primale jednaki volumen 0,9% NaCl. U intervalima od 24 sata, 4 tjedna i 6 tjedana analizirani su intraokularni tlak, funkcija zjeničnog sfinktera, krvne žile fundusa i disk vidnog živca te histološka prezentacija retine. Neinvazivno mjerenje očnog tlaka izvršeno je aplanacijskim tonometrom „Tonopen XL“. Snimanje zjenica mikrokamerom učinjeno je USB „Veho Discovery VMS-004 Deluxe“ te su polumjer (r) u mm, opseg (C) u mm i površina zjenica (S) u mm2 izmjereni posebnim softverom kupljenim s kamerom. Očna pozadina analizirana je mikrokamerom i „VOLK Digital Widefield“ 90D lupom te patohistološkom analizom bulbusa na standardnim rezovima, uklopljenog u parafin i bojenog hemalaun eozinom 24 sata, 4 tjedna i 6 tjedana nakon zahvata. Kauterizacija episkleralnih vena uzrokovala je povišenje očnog tlaka, proširenje zjenice, ishemiju očne pozadine i atrofiju diska vidnog živca. Intraperitonealna ili lokalna primjena BPC 157 dovela je do normalizacije očnog tlaka, oporavka zjeničnih funkcija, antagonizacije mrežnične ishemije te je spriječila atrofiju vidnog živca.

Abstract (english)

In female Wistar rats, 220 – 250 g, two dorsal and one temporal episcleral vein were isolated and cauterized. Medication was BPC 157 (10 μg/kg or 10 ng/kg) intraperitoneally (5 ml/kg) or locally (2x2 drops per day), either immediately before surgery and then once time daily; or at 24 h after surgery and then once time daily. Controls simultaneously received an equal volume of saline. Intraocular pressure, pupillar function were measured 24 hours, 4 and 6 weeks after procedure. Non-invasive IOP measurements were taken by aplanation tonometer “Tonopen XL”. Pupillar function was photographed by USB “Veho Discovery VMS-004 Deluxe” microcamera. Photographs were taken before and after medication application and analyzed with special camera software for measurement of pupillar diameter (r) in mm, range (C) in mm and surface (S) in mm2. Camera was previously calibrated using graph paper. Vascularization of the eye fundus and presentation of optic disc were analyzed with microcamera and “VOLK Digital Widefield lupe” 90D. Pathohistological analysis was performed after sacrifice. Episcleral vein cauterization caused high intraocular pressure, changes in pupillar function, retinal ischemia and optic nerve disk atrophy. BPC 157 applied locally or intraperitoneally normalized intraocular pressure, pupillar function, antagonized retinal ischemia and optic nerve atrophy.

• Keywords: medicina
• Keywords (english): medicine
• Language: croatian
• URN:NBN: urn:nbn:hr:105:569040
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstva (doktor/doktorica znanosti, područje biomedicine i zdravstva)
• Type of resource: Text
• Extent: 127 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-05-05 08:26:58