Novosti u laboratorijskoj dijagnostici malarije

master's thesis

• Title (english): Novelties in laboratory diagnostics of malaria
• Author: Viktor Bekić
• Mentor: Mario Sviben (mentor)
• Committee member: Ljiljana Žmak (predsjednik povjerenstva)
• Committee member: Goran Tešović (član povjerenstva)
• Committee member: Mario Sviben (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Medical Microbiology and Parasitology); Zagreb
• Defense date and country: 2023-09-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Medical Microbiology

Abstract

Malarija, kao jedan od vodećih svjetskih javnozdravstvenih problema, 2021. godine dovela je do 619 000 smrtnih slučajeva. Globalni utjecaj malarije mogao bi se smanjiti boljom kontrolom bolesti za koju je potrebna pravovremena i ispravna dijagnoza. Od otkrića samog uzročnika bolesti pa do danas, svjetlosna mikroskopija uzoraka krvi i dalje ostaje zlatni standard u dijagnostici, no njome je teško odrediti bolesti s niskom razinom parazitemije što otežava nastojanja njene eradikacije. Shodno tome, razvijaju se brojne nove imunokromatografske i molekularne metode u borbi protiv malarije. Integracijom laboratorijskih metoda na prijenosne uređaje koji bi omogućili primjenu na terenu nastoji se pružiti bolji pristup malariji u područjima s ograničenim resursima. Ovaj pregledni rad osvrće se na laboratorijske metode korištene u rutinskoj dijagnostici, na nove eksperimentalne metode tek u nastajanju, kao i na biologiju uzročnika Plasmodium spp., čije je poznavanje nužno za optimizaciju dijagnostičkih metoda.

Abstract (english)

Malaria, as one of the leading global public health challenges, lead to 619,000 deaths in 2021. The global impact of malaria could be reduced through improved disease control, which requires timely and accurate diagnosis. Since the discovery of its causative agent, light microscopy of blood samples has remained the gold standard for diagnostics. However, it faces challenges in detecting infections with low parasitemia, making eradication efforts difficult. Hence, numerous new immunochromatographic and molecular methods are being developed in the fight against malaria. Integrating laboratory methods into portable devices for field application aims to provide better access to malaria diagnostics in resource-limited areas. This review focuses on laboratory methods used in routine diagnostics, emerging experimental approaches, along with the biology of Plasmodium spp., knowledge of which is crucial for optimizing diagnostic methods.

• Keywords: malarija; dijagnostika; laboratorijske metode; molekularna dijagnostika; Plasmodium spp.
• Keywords (english): malaria; diagnostics; laboratory methods; molecular diagnostics; Plasmodium spp.
• Language: croatian
• URN:NBN: urn:nbn:hr:105:237920
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-10-20 11:31:14