Toksikološka procjena nanočestica zlata u ulozi nosača L-Dope na in vitro modelu diferenciranih SH-SY5Y stanica

master's thesis

• Title (english): Toxicological assessment of gold nanoparticles in the role od L-Dope carriers in the in vitro model of differentiated SH-SY5Y cells
• Author: Manuela Frančić
• Mentor: Krešimir Rotim (mentor)
• Committee member: Davor Mijatović (predsjednik povjerenstva)
• Committee member: Anko Antabak (član povjerenstva)
• Committee member: Krešimir Rotim (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Surgery); Zagreb
• Defense date and country: 2023-07-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Surgery

Abstract

Primjena nanočestica zlata za dostavu L-Dope mogla bi se pokazati kao inovativna terapija Parkinsonove bolesti. No, prije ispitivanja njihove učinkovitosti, važno je utvrditi kompatibilnost s biološkim sustavima. Na modelu stanica sličnih dopaminergičkim neuronima ispitivan je utjecaj AuNP na vijabilnost i oksidativni stres, kao i moguće zaštitno djelovanje. Model stanica sličnih dopaminergičkim neuronima dobiven je RA/TPA diferencijacijom SH-SY5Y stanica što je potvrđeno qPCR-om i imunocitokemijom. Unos AuNP u stance određen je imunocitokemijom i protočnom citometrijom kojom su analizirane i stanična vijabilnost i apoptoza. Oksidativni stres istražen je mjerenjem razine reaktivnih kisikovih vrsta, koncentracije glutationa i potencijala mitohondrijske membrane.

Abstract (english)

The use of gold nanoparticles to deliver L-Dopa could prove to be an innovative therapy for Parkinson’s disease. But before examining their effectiveness, it is important to determine compatibility with biological systems. The effect of AuNP on viability and oxidative stress, as well as possible protective effects, was investigated in a cell model of dopaminergic neuron-like cells. A cell model similar to dopaminergic neurons was obtained by RA / TPA differentiation of SH-SY5Y cells as confirmed by qPCR and immunocytochemistry. The uptake of AuNP into cells was determined by immunocytochemistry and flow cytometry which analyzed both cell viability and apoptosis. Oxidative stress was investigated by measuring the level of reactive oxygen species, glutathione concentration, and mitochondrial membrane potential.

• Keywords: nanočestice zlata; oksidativni stres; Parkinsonova bolest
• Keywords (english): gold nanoparticles; oxidative stress; Parkinson's disease
• Language: croatian
• URN:NBN: urn:nbn:hr:105:194065
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-10-24 09:57:28