prikaz prve stranice dokumenta In vivo fate mapping identifies mesenchymal progenitor cells
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Scientific paper - Original scientific paper
In vivo fate mapping identifies mesenchymal progenitor cells
Stem Cells, 30 (2012), 2; 187-196. https://doi.org/10.1002/stem.780
Grčević, Danka; Pejda, Slavica; Matthews, Brya G.; Repić, Dario; Wang, Liping; Li, Haitao; Kronenberg, Mark S.; Jiang, Xi; Maye, Peter; Adams, Douglas J.; Rowe, David W.; Aguila, Hector L.; Kalajzić, Ivo

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Grčević, D., Pejda, S., Matthews, B. G., Repić, D., Wang, L., Li, H. ... Kalajzić, I. (2012). In vivo fate mapping identifies mesenchymal progenitor cells. Stem Cells, 30. (2), 187-196. doi: 10.1002/stem.780

Grčević, Danka, et al. "In vivo fate mapping identifies mesenchymal progenitor cells." Stem Cells, vol. 30, no. 2, 2012, pp. 187-196. https://doi.org/10.1002/stem.780

Grčević, Danka, Slavica Pejda, Brya G. Matthews, Dario Repić, Liping Wang, Haitao Li, Mark S. Kronenberg, et al. "In vivo fate mapping identifies mesenchymal progenitor cells." Stem Cells 30, no. 2 (2012): 187-196. https://doi.org/10.1002/stem.780

Grčević, D., et al. (2012) 'In vivo fate mapping identifies mesenchymal progenitor cells', Stem Cells, 30(2), pp. 187-196. doi: 10.1002/stem.780

Grčević D, Pejda S, Matthews BG, Repić D, Wang L, Li H, and sur.. In vivo fate mapping identifies mesenchymal progenitor cells. Stem Cells [Internet]. 2012 February [cited 2024 November 09];30(2):187-196. doi: 10.1002/stem.780

D. Grčević, et al., "In vivo fate mapping identifies mesenchymal progenitor cells", Stem Cells, vol. 30, no. 2, pp. 187-196, February 2012. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:216677. [Accessed: 09 November 2024]

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Metadata
Title (english)In vivo fate mapping identifies mesenchymal progenitor cells
AuthorDanka Grčević
AuthorSlavica Pejda
AuthorBrya G. Matthews
AuthorDario Repić
AuthorLiping Wang
AuthorHaitao Li
AuthorMark S. Kronenberg
AuthorXi Jiang
AuthorPeter Maye
AuthorDouglas J. Adams
AuthorDavid W. Rowe
AuthorHector L. Aguila
AuthorIvo Kalajzić
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Adult mesenchymal progenitor cells have enormous potential for use in regenerative medicine. However, the true identity of the progenitors in vivo and their progeny has not been precisely defined. We hypothesize that cells expressing a smooth muscle α-actin promoter (αSMA)-directed Cre transgene represent mesenchymal progenitors of adult bone tissue. By combining complementary colors in combination with transgenes activating at mature stages of the lineage, we characterized the phenotype and confirmed the ability of isolated αSMA(+) cells to progress from a progenitor to fully mature state. In vivo lineage tracing experiments using a new bone formation model confirmed the osteogenic phenotype of αSMA(+) cells. In vitro analysis of the in vivo-labeled SMA9(+) cells supported their differentiation potential into mesenchymal lineages. Using a fracture-healing model, αSMA9(+) cells served as a pool of fibrocartilage and skeletal progenitors. Confirmation of the transition of αSMA9(+) progenitor cells to mature osteoblasts during fracture healing was assessed by activation of bone-specific Col2.3emd transgene. Our findings provide a novel in vivo identification of defined population of mesenchymal progenitor cells with active role in bone remodeling and regeneration.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleStem Cells
Numberingvol. 30, no. 2, pp. 187-196
p-ISSN1066-5099
DOIhttps://doi.org/10.1002/stem.780
URN:NBNurn:nbn:hr:105:216677
Publication2012-02
Document URLhttps://doi.org/10.1002/stem.780
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-27 19:29:35