Increased expression of endothelial lipase in symptomatic and unstable carotid plaques

Scientific paper - Original scientific paper

• Author: Matias Trbušić
• Author: Monika Riederer
• Author: Majda Vučić
• Author: Ivo Lovričević
• Author: Božo Krušlin
• Author: Martin Gauster
• Author: Sonja Mohrenz
• Author: Andrea Berghold
• Author: Beate Tiran
• Author: Vesna Degoricija
• Author: Saša Frank
• Author's institution: University of Zagreb; School of Medicine
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences

Abstract (english)

The aim of this study was to evaluate endothelial lipase (EL) protein expression in advanced human carotid artery plaques (HCAP) with regard to plaque (in)stability and the incidence of symptoms. HCAP were collected from 66 patients undergoing carotid endarterectomy (CEA). The degree of plaque (in)stability was estimated by ultrasound and histology. In HCAP sections, EL expression was determined by immunostaining and the intensity was assessed on a semi-quantitative scale (low: <25%, high: >25% positive cells). Monocytes and macrophages in adjacent HCAP sections were stained with a CD163 specific antibody. High EL staining was more prevalent in histologically unstable plaques (in 33.3% of fibrous plaques, 50% of ulcerated non-complicated plaques and 79.2% of ulcerated complicated plaques; χ(2) test, p = 0.004) and in the symptomatic group (70.8 vs. 42.9% in the asymptomatic group; χ(2) test, p = 0.028). The majority of EL immunostaining was found in those HCAP regions exhibiting a strong CD163 immunostaining. EL in HCAP might be a marker and/or promoter of plaque instability and HCAP-related symptomatology.

• Keywords (english): Adult; Aged; Aged, 80 and over; Antigens, CD/metabolism; Antigens, Differentiation, Myelomonocytic/metabolism; Body Mass Index; Carotid Arteries/pathology; Carotid Stenosis/complications; Carotid Stenosis/pathology; Carotid Stenosis/surgery; Echocardiography, Doppler, Color/methods; Endarterectomy, Carotid/methods; Endothelial Cells/enzymology; Female; Gene Expression Regulation, Enzymologic/physiology; Humans; Lipase/metabolism; Magnetic Resonance Angiography; Male; Middle Aged; Receptors, Cell Surface/metabolism; Renin/metabolism; Retrospective Studies
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Accepted version
• Journal title: Journal of Neurology
• Numbering: vol. 259, no. 3, pp. 448-456
• p-ISSN: 0340-5354
• DOI: https://doi.org/10.1007/s00415-011-6198-3
• URN:NBN: urn:nbn:hr:105:659357
• Publication: 2012-03
• Document URL: https://doi.org/10.1007/s00415-011-6198-3
• Type of resource: Text
• Access conditions: Open access
• Created on: 2024-03-27 19:32:51