prikaz prve stranice dokumenta Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells
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Scientific paper - Original scientific paper
Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells
Food and Chemical Toxicology, 107 (2017), Pt. A; 349-361. https://doi.org/10.1016/j.fct.2017.07.016
Brkić Ahmed, Lada; Milić, Mirta; Pongrac, Igor M.; Marjanović, Ana Marija; Mlinarić, Hrvoje; Pavičić, Ivan; Gajović, Srećko; Vinković Vrček, Ivana

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Brkić Ahmed, L., Milić, M., Pongrac, I. M., Marjanović, A. M., Mlinarić, H., Pavičić, I. ... Vinković Vrček, I. (2017). Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells. Food and Chemical Toxicology, 107. (Pt. A), 349-361. doi: 10.1016/j.fct.2017.07.016

Brkić Ahmed, Lada, et al. "Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells." Food and Chemical Toxicology, vol. 107, no. Pt. A, 2017, pp. 349-361. https://doi.org/10.1016/j.fct.2017.07.016

Brkić Ahmed, Lada, Mirta Milić, Igor M. Pongrac, Ana Marija Marjanović, Hrvoje Mlinarić, Ivan Pavičić, Srećko Gajović and Ivana Vinković Vrček. "Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells." Food and Chemical Toxicology 107, no. Pt. A (2017): 349-361. https://doi.org/10.1016/j.fct.2017.07.016

Brkić Ahmed, L., et al. (2017) 'Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells', Food and Chemical Toxicology, 107(Pt. A), pp. 349-361. doi: 10.1016/j.fct.2017.07.016

Brkić Ahmed L, Milić M, Pongrac IM, Marjanović AM, Mlinarić H, Pavičić I, and sur.. Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells. Food and Chemical Toxicology [Internet]. 2017 September [cited 2025 February 20];107(Pt. A):349-361. doi: 10.1016/j.fct.2017.07.016

L. Brkić Ahmed, et al., "Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells", Food and Chemical Toxicology, vol. 107, no. Pt. A, pp. 349-361, September 2017. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:037776. [Accessed: 20 February 2025]

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Title (english)Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells
AuthorLada Brkić Ahmed
AuthorMirta Milić
AuthorIgor M. Pongrac
AuthorAna Marija Marjanović
AuthorHrvoje Mlinarić
AuthorIvan Pavičić
AuthorSrećko Gajović
AuthorIvana Vinković Vrček
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Safe and successful bioapplications of metallic nanoparticles depend on their physicochemical characteristics, in particular their surface properties. This study aimed to investigate how different surface functionalization of silver nanoparticles (AgNP) affect their interaction with mammalian liver cells with regard to cytotoxicity, genotoxicity and mechanism of cellular uptake. Differentially coated AgNP were prepared by surface functionalization using sodium bis(2-ethylhexyl)-sulfosuccinate (AOTAgNP), cetyltrimethylammonium bromide (CTABAgNP), poly(vinylpyrrolidone) (PVPAgNP), poly-l-lysine (PLLAgNP), and bovine serum albumin (BSAAgNP). Data showed varying toxic potential of differentially coated AgNP. All AgNP types demonstrated concentration dependent effects on cytotoxicity and genotoxicity in HepG2 cells. Cytotoxic potential of differentially coated AgNP followed the order of BSAAgNP > PLLAgNP > CTABAgNP > AOTAgNP > PVPAgNP. Exposure of HepG2 cells to non-cytotoxic concentrations (up to 10 mg Ag/L) of AgNP for 24 h induced primary DNA damage as evaluated by alkaline comet assay. The highest increase in both comet tail length and tail intensity was produced by PLLAgNP followed by AOTAgNP, while CTABAgNP appeared to be least damaging. The main uptake mechanisms of AgNP were macropinocytosis and clathrin-mediated endocytosis. The study findings contribute to the criteria that should be considered in evaluating the biocompatibility and safety of novel nanomaterials.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionAccepted version
Journal titleFood and Chemical Toxicology
Numberingvol. 107, no. Pt. A, pp. 349-361
p-ISSN0278-6915
DOIhttps://doi.org/10.1016/j.fct.2017.07.016
URN:NBNurn:nbn:hr:105:037776
Publication2017-09
Document URLhttps://doi.org/10.1016/j.fct.2017.07.016
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 09:13:52