prikaz prve stranice dokumenta BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways
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Scientific paper - Original scientific paper
BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways
International Orthopaedics, 31 (2007), 6; 759-765. https://doi.org/10.1007/s00264-007-0407-9
Grasser, William A.; Orlić, Iva; Borovečki, Fran; Riccardi, K. A.; Šimić, Petra; Vukičević, Slobodan; Paralkar, Vishwas M.

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Grasser, W. A., Orlić, I., Borovečki, F., Riccardi, K. A., Šimić, P., Vukičević, S. & Paralkar, V. M. (2007). BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways. International Orthopaedics, 31. (6), 759-765. doi: 10.1007/s00264-007-0407-9

Grasser, William A., et al. "BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways." International Orthopaedics, vol. 31, no. 6, 2007, pp. 759-765. https://doi.org/10.1007/s00264-007-0407-9

Grasser, William A., Iva Orlić, Fran Borovečki, K. A. Riccardi, Petra Šimić, Slobodan Vukičević and Vishwas M. Paralkar. "BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways." International Orthopaedics 31, no. 6 (2007): 759-765. https://doi.org/10.1007/s00264-007-0407-9

Grasser, W. A., et al. (2007) 'BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways', International Orthopaedics, 31(6), pp. 759-765. doi: 10.1007/s00264-007-0407-9

Grasser WA, Orlić I, Borovečki F, Riccardi KA, Šimić P, Vukičević S, and sur.. BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways. International Orthopaedics [Internet]. 2007 December [cited 2024 December 21];31(6):759-765. doi: 10.1007/s00264-007-0407-9

W. A. Grasser, et al., "BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways", International Orthopaedics, vol. 31, no. 6, pp. 759-765, December 2007. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:674422. [Accessed: 21 December 2024]

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Metadata
Title (english)BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways
AuthorWilliam A. Grasser
AuthorIva Orlić
AuthorFran Borovečki
AuthorK. A. Riccardi
AuthorPetra Šimić
AuthorSlobodan Vukičević
AuthorVishwas M. Paralkar
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
We have recently shown that human recombinant BMP-6 (rhBMP-6), given systematically, can restore bone in animal models of osteoporosis. To further elucidate the underlying mechanisms of new bone formation following systemic application of BMPs, we conducted gene expression profiling experiments using bone samples of oophrectomised mice treated with BMP-6. Gene set enrichment analysis revealed enrichment of insulin-like growth factor-I and epidermal growth factor related pathways in animals treated with BMP-6. Significant upregulation of IGF-I and EGF expression in bones of BMP-6 treated mice was confirmed by quantitative PCR. To develop an in vitro model for evaluation of the effects of BMP-6 on cells of human origin, we cultured primary human osteoblasts. Treatment with rhBMP-6 accelerated cell differentiation as indicated by the formation of mineralised nodules by day 18 of culture versus 28-30 days in vehicle treated cultures. In addition, alkaline phosphatase gene expression and activity were dramatically increased upon BMP-6 treatment. Expression of IGF-I and EGF was upregulated in human osteoblast cells treated with BMP-6. These results collectively indicate that BMP-6 exerts its osteoinductive effect, at least in part, through IGF-I and EGF pathways, which can be observed both in a murine model of osteopenia and in human osteoblasts.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionAccepted version
Journal titleInternational Orthopaedics
Numberingvol. 31, no. 6, pp. 759-765
p-ISSN0341-2695
DOIhttps://doi.org/10.1007/s00264-007-0407-9
URN:NBNurn:nbn:hr:105:674422
Publication2007-12
Document URLhttps://doi.org/10.1007/s00264-007-0407-9
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 09:27:16