prikaz prve stranice dokumenta Different behaviour of DVL1, DVL2, DVL3 in astrocytoma malignancy grades and their association to TCF1 and LEF1 upregulation
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Scientific paper - Original scientific paper
Different behaviour of DVL1, DVL2, DVL3 in astrocytoma malignancy grades and their association to TCF1 and LEF1 upregulation
Journal of Cellular and Molecular Medicine, 23 (2018), 1; 641-655. https://doi.org/10.1111/jcmm.13969
Kafka, Anja; Bačić, Mateja; Tomas, Davor; Žarković, Kamelija; Bukovac, Anja; Njirić, Niko; Mrak, Goran; Krsnik, Željka; Pećina-Šlaus, Nives

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Kafka, A., Bačić, M., Tomas, D., Žarković, K., Bukovac, A., Njirić, N. ... Pećina Šlaus, N. (2018). Different behaviour of DVL1, DVL2, DVL3 in astrocytoma malignancy grades and their association to TCF1 and LEF1 upregulation. Journal of Cellular and Molecular Medicine, 23. (1), 641-655. doi: 10.1111/jcmm.13969

Kafka, Anja, et al. "Different behaviour of DVL1, DVL2, DVL3 in astrocytoma malignancy grades and their association to TCF1 and LEF1 upregulation." Journal of Cellular and Molecular Medicine, vol. 23, no. 1, 2018, pp. 641-655. https://doi.org/10.1111/jcmm.13969

Kafka, Anja, Mateja Bačić, Davor Tomas, Kamelija Žarković, Anja Bukovac, Niko Njirić, Goran Mrak, Željka Krsnik and Nives Pećina Šlaus. "Different behaviour of DVL1, DVL2, DVL3 in astrocytoma malignancy grades and their association to TCF1 and LEF1 upregulation." Journal of Cellular and Molecular Medicine 23, no. 1 (2018): 641-655. https://doi.org/10.1111/jcmm.13969

Kafka, A., et al. (2018) 'Different behaviour of DVL1, DVL2, DVL3 in astrocytoma malignancy grades and their association to TCF1 and LEF1 upregulation', Journal of Cellular and Molecular Medicine, 23(1), pp. 641-655. doi: 10.1111/jcmm.13969

Kafka A, Bačić M, Tomas D, Žarković K, Bukovac A, Njirić N, and sur.. Different behaviour of DVL1, DVL2, DVL3 in astrocytoma malignancy grades and their association to TCF1 and LEF1 upregulation. Journal of Cellular and Molecular Medicine [Internet]. 2018 January [cited 2024 December 01];23(1):641-655. doi: 10.1111/jcmm.13969

A. Kafka, et al., "Different behaviour of DVL1, DVL2, DVL3 in astrocytoma malignancy grades and their association to TCF1 and LEF1 upregulation", Journal of Cellular and Molecular Medicine, vol. 23, no. 1, pp. 641-655, January 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:471426. [Accessed: 01 December 2024]

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Title (english)Different behaviour of DVL1, DVL2, DVL3 in astrocytoma malignancy grades and their association to TCF1 and LEF1 upregulation
AuthorAnja Kafka
AuthorMateja Bačić
AuthorDavor Tomas
AuthorKamelija Žarković
AuthorAnja Bukovac
AuthorNiko Njirić
AuthorGoran Mrak
AuthorŽeljka Krsnik
AuthorNives Pećina-Šlaus
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Key regulators of the Wnt signalling, DVL1, DVL2 and DVL3, in astrocytomas of different malignancy grades were investigated. Markers for DVL1, DVL2 and DVL3 were used to detect microsatellite instability (MSI) and gross deletions (LOH), while immunohistochemistry and immunoreactivity score were used to determine the signal strengths of the three DVL proteins and transcription factors of the pathway, TCF1 and LEF1. Our findings demonstrated that MSI at all three DVL loci was constantly found across tumour grades with the highest number in grade II (P = 0.008). Collectively, LOHs were more frequent in high-grade tumours than in low grade ones. LOHs of DVL3 gene were significantly associated with grade IV tumours (P = 0.007). The results on protein expressions indicated that high-grade tumours expressed less DVL1 protein as compared with low grade ones. A significant negative correlation was established between DVL1 expression and malignancy grades (P < 0.001). The expression of DVL2 protein was found similar across grades, while DVL3 expression significantly increased with malignancy grades (P < 0.001). The signal strengths of expressed DVL1 and DVL3 were negatively correlated (P = 0.002). However, TCF1 and LEF1 were both significantly upregulated and increasing with astrocytoma grades (P = 0.001). A positive correlation was established between DVL3 and both TCF1 (P = 0.020) and LEF1 (P = 0.006) suggesting their joint involvement in malignant progression. Our findings suggest that DVL1 and DVL2 may be involved during early stages of the disease, while DVL3 may have a role in later phases and together with TCF1 and LEF1 promotes the activation of Wnt signalling.
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleJournal of Cellular and Molecular Medicine
Numberingvol. 23, no. 1, pp. 641-655
p-ISSN1582-1838
DOIhttps://doi.org/10.1111/jcmm.13969
URN:NBNurn:nbn:hr:105:471426
Publication2018-01
Document URLhttps://doi.org/10.1111/jcmm.13969
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 09:55:33