Fluid perfusion as a method of cerebrospinal fluid formation rate - critical appraisal

Scientific paper - Original scientific paper

• Author: Darko Orešković
• Author: Jurica Maraković
• Author: Miroslav Vukić
• Author: Milan Radoš
• Author: Marijan Klarica
• Author's institution: University of Zagreb; School of Medicine
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences

Abstract (english)

The aim of the study was to evaluate whether or not cerebrospinal fluid formation rate (Vf) calculated according to the equation of Heisey et al., truly show the produced cerebrospinal fluid. For this reason Vf was simulated (40.6 microL/min) by an infusion pump in a plastic cylinder and the evaluation was done by comparing the results obtained between the calculated Vf and the simulated one. In both cases the result should be the same (40.6 micro/min). Other types of experiments were carried out by ventriculocisternal perfusion (92.4 microL/min) on anaesthetized and sacrificed cats. If the equation is correct, the calculated Vf for sacrificed animals should be zero, because there is no Vf in dead animals. The fact that the calculated Vf (46.5 microL/min) in the plastic cylinder was different (p < 0.0001) from the simulated one (40.6 microL/min) and that Vf was calculated even for dead animals (3-5 microL/min) clearly shows the that perfusion method may not be an accurate method for determination of Vf.

Abstract (croatian)

Svrha prikazanih istraživanja bila je provjeriti da li perfuziona metoda izračunavanja stvaranja cerebrospinalnog likvora (Vf) po Heiseyu i sur. zaista prikazuje stvaranje likvora. U tu svrhu »Vf« je u plastičnoj komorici simuliran pomoću infuzione pumpe (40,6 mL/min), a provjera je vršena uspoređivanjem rezultata izračunanog Vf-a s pomoću Heiseyeve perfuzione metode i onog simuliranog s pomoću pumpe. U slučaju da je prefusiona metoda točna, rezultat bi trebao biti u oba slučaja isti (40,6 mL/min). U drugoj skupini pokusa vršena je ventrikulocisternalna perfuzija (brzina 92,4 mL/min) na anesteziranim i žrtvovanim mačkama. Ukoliko je ispitivana perfuziona metoda točna izračunane vrijednosti na žrtvovanim mačkama Vf-a bi morale iznositi 0 mL/min, jer ne postoji stvaranje likvora na mrtvim životinjama. Budući da se izračunani »Vf« (46,5 mL/min) u plastičnoj komorici značajno razlikuje (p<0.0001) od simuliranog s pomoću pumpe (40,6 mL/min) i budući da je izračunani Vf kod mrtvih životinja varirao od 3–5 mL/min, onda to jasno ukazuje da se perfuziona metoda ne može koristiti kao pouzdana metoda za izračunavanje stvaranja cerebrospinalnog likvora (Vf).

• Keywords (english): Cerebral Ventricles - physiology; Cerebrospinal Fluid - physiology; Models, Biological; Animals; Cats; Female; Male; Perfusion
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: Collegium Antropologicum
• Numbering: vol. 32, no. S. 1, pp. 133-137
• p-ISSN: 0350-6134
• URN:NBN: urn:nbn:hr:105:127589
• Publication: 2008-01
• Document URL: https://hrcak.srce.hr/24567
• Type of resource: Text
• Access conditions: Open access
• Created on: 2024-03-28 10:47:21