prikaz prve stranice dokumenta Emergence of uropathogenic extended-spectrum beta lactamases-producing Escherichia coli strains in the community
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Scientific paper - Original scientific paper
Emergence of uropathogenic extended-spectrum beta lactamases-producing Escherichia coli strains in the community
Collegium Antropologicum, 31 (2007), 1; 227-233. urn:nbn:hr:105:645016
Marijan, Tatjana; Vraneš, Jasmina; Bedenić, Branka; Mlinarić-Džepina, Ana; Plečko, Vanda; Kalenić, Smilja

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Marijan, T., Vraneš, J., Bedenić, B., Mlinarić Džepina, A., Plečko, V. & Kalenić, S. (2007). Emergence of uropathogenic extended-spectrum beta lactamases-producing Escherichia coli strains in the community. Collegium Antropologicum, 31. (1), 227-233. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:645016

Marijan, Tatjana, et al. "Emergence of uropathogenic extended-spectrum beta lactamases-producing Escherichia coli strains in the community." Collegium Antropologicum, vol. 31, no. 1, 2007, pp. 227-233. https://urn.nsk.hr/urn:nbn:hr:105:645016

Marijan, Tatjana, Jasmina Vraneš, Branka Bedenić, Ana Mlinarić Džepina, Vanda Plečko and Smilja Kalenić. "Emergence of uropathogenic extended-spectrum beta lactamases-producing Escherichia coli strains in the community." Collegium Antropologicum 31, no. 1 (2007): 227-233. https://urn.nsk.hr/urn:nbn:hr:105:645016

Marijan, T., et al. (2007) 'Emergence of uropathogenic extended-spectrum beta lactamases-producing Escherichia coli strains in the community', Collegium Antropologicum, 31(1), pp. 227-233. Available at: https://urn.nsk.hr/urn:nbn:hr:105:645016 (Accessed 13 January 2025)

Marijan T, Vraneš J, Bedenić B, Mlinarić Džepina A, Plečko V, Kalenić S. Emergence of uropathogenic extended-spectrum beta lactamases-producing Escherichia coli strains in the community. Collegium Antropologicum [Internet]. 2007 March [cited 2025 January 13];31(1):227-233. Available at: https://urn.nsk.hr/urn:nbn:hr:105:645016

T. Marijan, J. Vraneš, B. Bedenić, A. Mlinarić Džepina, V. Plečko and S. Kalenić, "Emergence of uropathogenic extended-spectrum beta lactamases-producing Escherichia coli strains in the community", Collegium Antropologicum, vol. 31, no. 1, pp. 227-233, March 2007. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:645016. [Accessed: 13 January 2025]

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Metadata
Title (english)Emergence of uropathogenic extended-spectrum beta lactamases-producing Escherichia coli strains in the community
AuthorTatjana Marijan
AuthorJasmina Vraneš
AuthorBranka Bedenić
AuthorAna Mlinarić-Džepina
AuthorVanda Plečko
AuthorSmilja Kalenić
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
The aim of this study was to determine the virulence characteristics and resistance pattern of the extended-spectrum/lactamases (ESBLs)-producing Escherichia coli strains isolated from urine of outpatients in the Zagreb region during a five-month period, and to compare them with the non ESBLs-producing E. coli strains isolated in the same period. Out of 2451 E. coli strains isolated from urine of nonhospitalized patients with significant bacteriuria, a total of 39 ESBLs-producing strains (1.59%) were detected by a double-disk diffusion technique and by the broth-dilution minimal inhibitory concentration reduction method. The 45 non ESBLs-producing strains were randomly chosen, and phenotype of the two groups of strains was characterized and compared. Serogroup O4, hemolysin production, expression of P- and type 1 fimbriae as well as resistance to gentamicin and amikacin were significantly more prevalent characteristics among the ESBLs-producing strains than among non ESBLs-producing strains (p < 0.01), while higher prevalence of trimethoprim-sulfamethoxazole resistance among ESBLs-producing strains was not statistically significant (p > 0.05). Chromosomal DNA analysis by pulsed-field gel electrophoresis exhibited a great genomic similarity among ESBLs-producing strains and revealed that those highly virulent and resistant E. coli strains isolated from urine of outpatients in the Zagreb region had a clonal propagation.
Abstract (croatian)
Cilj ovog istraživanja bio je utvrditi virulentne značajke i rezistotip sojeva bakterije Escherichia coli koji produciraju β laktamaze proširenog spektra, izoliranih iz mokraće ambulantnih pacijenata zagrebačke regije u razdoblju od pet mjeseci, i usporediti ih sa sojevima E. coli koji ne stvaraju β laktamaze proširenog spektra, a izolirani su u istom razdoblju. Od 2451 E. coli sojeva izoliranih iz mokraće izvanbolničkih pacijenata sa signifikantnom bakteriurijom, ukupno 39 sojeva produciralo je β laktamaze proširenog spektra (1,59%), što je detektirano metodom dvostruke disk-difuzije i metodom redukcije minimalne inhibicijske koncentracije u bujonu. Slučajnim odabirom prikupljeno je 45 sojeva koji ne produciraju b laktamaze proširenog spektra, te je određen i uspoređen fenotip ove dvije grupe sojeva. Najzastupljenije značajke sojeva koji su producirali β laktamaze proširenog spektra bile su serogrupa O4, produkcija hemolizina, ispoljavanje P i tip 1 fimbrija, te rezistencija na gentamicin i amikacin (p<0,01), dok veća zastupljenost rezistencije na trimetoprim-sulfametoksazol nije bila statistički značajna (p>0,05). Kromosomska DNA analiza pomoću gel-elektroforeze u izmjeničnom polju pokazala je veliku genetsku sličnost među sojevima koji produciraju β laktamaze proširenog spektra i otkrila klonalno širenje ovih visoko virulentnih i rezistentnih uropatogenih sojeva E. coli među izvanbolničkim pacijentima zagrebačke regije.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleCollegium Antropologicum
Numberingvol. 31, no. 1, pp. 227-233
p-ISSN0350-6134
URN:NBNurn:nbn:hr:105:645016
Publication2007-03
Document URLhttps://hrcak.srce.hr/27312
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 10:56:20