Persistant immunodominant anti-gag SLYNTVATL responses in HIV-patients with up to 7 years of HAART

Scientific paper - Original scientific paper

• Author: Snježana Židovec Lepej
• Author: Ela Kosor
• Author: Alenka Gagro
• Author: Adriana Vince
• Author: Anica Remenar
• Author: Mario Poljak
• Author's institution: University of Zagreb; School of Medicine
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences

Abstract (english)

We analyzed Gag-specific CD8+ T-cells in HIV-patients on long-term HAART and in untreated chronically-infected patients by using iTAg MHC class I tetramers (HLA-A*0201) specific for SLYNTVATL. Gag SLYNTVATL-specific CD8+ T-cells were detectable in 18 of 26 treated patients (median 5.2 years of HAART) and in 10 of 14 untreated patients. Median percentage of Gag SLYNTVATL-specific CD8+ T-cells in treated patients was 0.10 (range 0.00-0.70%). Median number of Gag SLYNTVATL-specific CD8+ T-cells per 50,000 CD8+ T-cells was 56.0 cells (range 2.0-344.0 cells) and was not significantly different compared with untreated patients (p = 0.978). Numbers of Gag SLYNTVATL-specific CD8+ T-cells were inversely correlated with the duration of undetectable plasma viremia (p = 0.02, Rho = -0.430). Chronically-infected HIV-patients on HAART (for up to 7.7 years) maintained a stable subpopulation of Gag SLYNTVATL-specific CD8+ T-cells. This finding is relevant for the analysis of treatment-induced immune reconstitution and, possibly, for future therapeutic strategies in HIV-disease.

Abstract (croatian)

U ovom smo istraživanju analizirali Gag-specifične CD8+ T-limfocite u HIV-bolesnika liječenih HAART-om kao i u neliječenih zaraženih osoba primjenom iTAg MHC tetramera klase 1 (HLA-A*0201) specifičnih za SLYNTVATL. Gag SLYNTVATL-specifične CD8+ T-limfocite dokazali smo u 18 od 26 liječenih bolesnika (medijan 5,2 godine HAART-a) i u 10 od 14 neliječenih pacijenata. Medijan postotka Gag SLYNTVATL-specifičnih CD8+ T-limfocita u liječenih bolesnika bio je 0,10 (raspon 0,00–0,70%). Medijan broja Gag SLYNTVATL-specifičnih CD8+ T-limfocita na 50.000 CD8+ T-limfocita bio je 56,0 stanica (raspon 2,0–344,0 stanica) i nije bio značajno različit u odnosu na neliječene zaražene osobe (p=0,978). Broj Gag SLYNTVATL-specifičnih CD8+ T-limfocita negativno je korelirao s dužinom perioda nemjerljive viremije u plazmi (p=0,02, Rho= –0,430). U liječenih HIV-bolesnika (do 7,7 godina HAART-a) postoji stabilna populacija Gag SLYNTVATL specifičnih CD8+ T-limfocita. Rezultati ovog rada značajni su za analizu liječenjem-potaknute imunološke rekonstitucije te za razvoj novih strategija liječenja u HIV-bolesti.

• Keywords (english): Antiretroviral Therapy, Highly Active; Gene Products, gag - drug effects - immunology; HIV Infections - drug therapy - immunology; HIV-1 - immunology; HLA-A2 Antigen - blood - drug effects - immunology; Peptide Fragments - drug effects - immunology; Adult; Aged; Cross-Sectional Studies; Female; Humans; Male; Middle Aged; Prospective Studies; env Gene Products, Human Immunodeficiency Virus
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: Collegium Antropologicum
• Numbering: vol. 30, no. Suppl., pp. 33-38
• p-ISSN: 0350-6134
• URN:NBN: urn:nbn:hr:105:092867
• Publication: 2006-12
• Document URL: https://hrcak.srce.hr/27716
• Type of resource: Text
• Access conditions: Open access
• Created on: 2024-03-28 11:01:17