Human palatine tonsil: a new potential tissue source of multipotent mesenchymal progenitor cells

Scientific paper - Original scientific paper

• Author: Saša Janjanin
• Author: Farida Djouad
• Author: Rabie M. Shanti
• Author: Dolores Baksh
• Author: Kiran Gollapudi
• Author: Drago Prgomet
• Author: Lars Rackwitz
• Author: Arjun S. Joshi
• Author: Rocky S. Tuan
• Author's institution: University of Zagreb; School of Medicine
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences

Abstract (english)

INTRODUCTION: Mesenchymal progenitor cells (MPCs) are multipotent progenitor cells in adult tissues, for example, bone marrow (BM). Current challenges of clinical application of BM-derived MPCs include donor site morbidity and pain as well as low cell yields associated with an age-related decrease in cell number and differentiation potential, underscoring the need to identify alternative sources of MPCs. Recently, MPC sources have diversified; examples include adipose, placenta, umbilicus, trabecular bone, cartilage, and synovial tissue. In the present work, we report the presence of MPCs in human tonsillar tissue. ----- METHODS: We performed comparative and quantitative analyses of BM-MPCs with a subpopulation of adherent cells isolated from this lymphoid tissue, termed tonsil-derived MPCs (T-MPCs). The expression of surface markers was assessed by fluorescent-activated cell sorting analysis. Differentiation potential of T-MPCs was analyzed histochemically and by reverse transcription-polymerase chain reaction for the expression of lineage-related marker genes. The immunosuppressive properties of MPCs were determined in vitro in mixed lymphocyte reactions. ----- RESULTS: Surface epitope analysis revealed that T-MPCs were negative for CD14, CD31, CD34, and CD45 expression and positive for CD29, CD44, CD90, and CD105 expression, a characteristic phenotype of BM-MPCs. Similar to BM-MPCs, T-MPCs could be induced to undergo adipogenic differentiation and, to a lesser extent, osteogenic and chondrogenic differentiation. T-MPCs did not express class II major histocompatibility (MHC) antigens, and in a similar but less pronounced manner compared with BM-MPCs, T-MPCs were immunosuppressive, inhibiting the proliferation of T cells stimulated by allogeneic T cells or by non-specific mitogenic stimuli via an indoleamine 2,3-dioxygenase-dependent mechanism. ----- CONCLUSION: Human palatine T-MPCs represent a new source of progenitor cells, potentially applicable for cell-based therapies.

• Keywords (english): Adolescent; Adult; Biopsy; Bone Marrow Cells/cytology; Bone Marrow Cells/drug effects; Bone Marrow Cells/metabolism; Cell Proliferation; Cell Survival/physiology; Cells, Cultured; Child; Child, Preschool; Humans; Immunosuppression; Interferon-gamma/pharmacology; Lymphocyte Culture Test, Mixed; Mesenchymal Stem; Cells/cytology; Mesenchymal Stem Cells/drug effects; Mesenchymal Stem Cells/metabolism; Middle Aged; Multipotent Stem Cells/cytology; Multipotent Stem Cells/drug effects; Multipotent Stem Cells/metabolism; Palatine Tonsil/cytology; Palatine Tonsil/drug effects; Palatine Tonsil/metabolism; Receptors, Interferon/metabolism
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: Arthritis Research & Therapy
• Numbering: vol. 10, no. 4
• p-ISSN: 1478-6362
• DOI: https://doi.org/10.1186/ar2459
• URN:NBN: urn:nbn:hr:105:587189
• Publication: 2008
• Document URL: https://doi.org/10.1186/ar2459
• Type of resource: Text
• Access conditions: Open access
• Created on: 2024-03-28 11:08:46