Učinak koronavirusnih cjepiva BNT162b2 i Ad26.CoV2.S na proupalne i koagulacijske pokazatelje

doctoral thesis

• Title (english): The effect of BNT162b2 and Ad26.CoV2.S coronavirus vaccines on the parameters of hemostatis and inflammation
• Author: Iva Ivanko
• Mentor: Petar Gaćina (mentor)
• Mentor: Josipa Josipović (komentor)
• Committee member: Dražen Pulanić (predsjednik povjerenstva)
• Committee member: Robert Likić (član povjerenstva)
• Committee member: Ana Boban (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2024-05-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Internal Medicine
• Universal decimal classification (UDC): 616 - Pathology. Clinical medicine

Abstract

Glavni cilj ovog prospektivnog opservacijskog istraživanja bio je ispitati utjecaj mRNA BNT162b2 i vektorskog Ad26.CoV2.S cjepiva protiv SARS-CoV-2 na pokazatelje zgrušavanja i upale.
Istraživanje je obuhvatilo 87 ispitanika u mRNA skupini (prosječne dobi 35 god, udio žena 59% ) i 84 u vektorskoj skupini (prosječne dobi 44 godine, udio muškaraca 52%) kojima su određivani laboratorijski pokazatelji (TAT, F1+2, d-dimeri, IL-6, veliki ET-1, fibrinogen, VWF:Ac, CRP, KKS) prije, 7 i 14 dana nakon cijepljenja vektorskim, odnosno prije, 7 i 14 dana nakon 1. i 2. doze mRNA cjepiva.
Nakon cijepljenja zabilježen je porast vrijednosti d-dimera (P=0,004) i pad aktivnosti VWF-a u obje skupine (P<0,001 u) te porast CRP-a (P=0,006) u vektorskoj i velikog ET-1 (P<0,001) u mRNA grupi. Veći CRP (P=0,014) zabilježen je 7 dana nakon cijepljenja vektorskim cjepivom u odnosu na mRNA cjepivo. Kod svih ispitanika isključena je prisutnost protutijela na trombociti faktor 4.
Zaključno, statistički značajne razlike pokazatelja zgrušavanja i upale u našem istraživanju mogu se pripisati opće poznatoj i očekivanoj blagoj reakciji na cijepljenje SARS-CoV-2 cjepivima. Niti jedan ispitanik nije imao tromboembolijski incident u 6-mjesečnom periodu praćenja nakon cijepljenja.

Abstract (english)

The main aim of our study was to investigate the effect of mRNA BNT162b2 and vector Ad26.CoV2.S vaccine against SARS-CoV-2 on the markers of hemostasis and inflammation.
The study included 87 subjects in the mRNA group (median age 35 years, 59% of women) and 84 (median age 44 years, 52% of men) in the vector group. All the laboratory parameters (TAT, F1+2, d-dimers, VWF:Ac, fibrinogen, big ET-1, CRP, CBC) were investigated before, 7 and 14 days after the vaccination in the vector group and before, 7 and 14 day after the 1st and 2nd dose in the mRNA group.
After vaccination with both vaccines higher levels of d-dimers (P=0.004) and lower levels of VWF:Ac (P<0,001) were noted. CRP levels were eleveted (P=0.006) in the vector group while in the mRNA group, higer levels of big ET-1 (P<0,001) were noted. Higher CRP levels (P=0.014) were present 7 days after the vector vaccine immunisation in comparisson with the mRNA vaccine. All the study participants tested negative for platelet factor 4 antibodies.
In conclusion, although statistically significant changes in the parameters of hemostatasis and inflammation have been obtained, they remained clinically irrelevant (no thromoembolic events have been noted in the 6-months surveillance period) and can be contributed to the wellknown reaction to the vaccination.

• Keywords: Odrasla osoba; von Willebrandov faktor; Cjepivo BNT162; Covid-19 cjepiva; Trombocit faktor 4; SARS-CoV-2; mRNA cjepiva; Upala; Imunizacija; Cijepljenje; Fibrinogen; Hemostaza
• Keywords (english): Adult; von Willebrand Factor; BNT162 Vaccine; COVID-19 Vaccines; Platelet Factor 4; SARS-CoV-2; mRNA Vaccines; Inflammation; Immunization; Vaccination; Fibrinogen; Hemostasis
• Language: croatian
• URN:NBN: urn:nbn:hr:105:753928
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
• Type of resource: Text
• Extent: 96 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-05-16 08:07:45