Ekspresija ugljikohidrat (n-acetilglukozamin 6-0) sulfotransferaze 7 u adenokarcinomima i planocelularnim karcinomima pluća

master's thesis

• Title (english): Expression of carbohydrate (n-acetylglucosamine 6-0) sulfotransferase 7 in adenocarcinomas and squamouscell carcinomas of the lung
• Author: Luka Opašić
• Mentor: Danijela Kolenc (mentor)
• Committee member: Sven Seiwerth (predsjednik povjerenstva)
• Committee member: Slavko Gašparov (član povjerenstva)
• Committee member: Danijela Kolenc (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Pathology); Zagreb
• Defense date and country: 2015-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Pathology

Abstract

Karcinom pluća jedan je od najčešćih uzročnika smrti od karcinoma u modernom društvu. Iako je dio uzroka njegova nastanka relativno dobro objašnjen izlaganjem pojedinim okolišnim čimbenicima, u samoj njegovoj biologiji mnogo je nepoznanica. Odnos neoplastičnih stanica i strome izrazito je važan za maligno ponašanje karcinoma. Gen CHST7 sudjeluje u stvaranju matriksa i povezuje ga se sa biološkim ponašanjem nekih karcinoma i potencijalni je tumorski biljeg. Cilj ovog rada bio je istražiti ekspresiju CHST7 u najčešćim tipovima karcinoma pluća u odnosu na zdravo tkivo pluća različito eksprimiran u karcinomima pluća u odnosu na zdravo tkivo pluća. 40 parova uzoraka adenokarcinoma i planocelularnih karcinoma pluća s pripadajućim zdravim dijelom pluća podvrgnuto je qPCR analizi. Rezultati istraživanja nisu pokazali statistički značajnu razliku u ekspresiji gena CHST7 u karcinomima u odnosu na zdravo tkivo pluća. Rezultati nisu u skladu sa literaturom i sa predviđanjima. Dobiveni rezultat najvjerojatnije je posljedica odabira kontrolnog gena, ACTB, zbog toga jer njegova Cp vrijednost izrazito varira kod karcinoma i kod zdravog tkiva pluća. Budući korak je odabir više kontrolnih gena i ponovna analiza. Prema prikazanim rezultatima CHST7 nije pouzdan tumorski biljeg za karcinom pluća.

Abstract (english)

Lung carcinoma is one of the most common causes of death from carcinoma in modern societies. Although cause of it's development is partialiy explained by exposure to certain environmental agenses, much of it's biology is so far poorly understood. Interaction between tumor cells and it's extracellular matrix is very important for progression of carcinoma. CHST7 gene important in development of extracellular matrix and is associated with biological behaviour of some carcinomas, and is regarded as potential biomarker. Aim of this study was to investigate differential gene expression of CHST7 in lung adeno and squamous cell carcinoma compared to healty lung tissue. 40 pairs of lung adenocarcinoma and lung squamous cell carcinoma with matched healty lung tissue specimens were analysed with qPCR. Resultsdid not show statistically significant difference in gene expression. Results are not in accordance with previously published data. This is most probably due to great variability of ACTB gene's Cp value, both in carcinomas and in normal lung parenchyma. Further step is selection of another housekeeping gene and repetition of analysis. According to our results CHST7 is not reliable tumor marker for adenocarcinoma nor for squamous cell carcinoma of the lung.

• Keywords: karcinom pluća; genska ekspresija; tumorska stroma; CHST7
• Keywords (english): lung carcinoma; gene expression; extracellular matrix; CHST7
• Language: croatian
• URN:NBN: urn:nbn:hr:105:734561
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2016-12-07 12:18:15