Status F2 izoprostana, malondialdehida, vitamina E i superoksid-dismutaze u folikularnoj tekućini ispitanica s idiopatskom neplodnosti

doctoral thesis

• Title (english): Assessment of F2 isoprostane, malondialdehyde, vitamin E, and superoxide dismutase levels in follicular fluid of women with idiopathic infertility
• Author: Ivana Zec
• Mentor: Marina Šprem-Goldštajn (mentor)
• Mentor: Krunoslav Kuna (komentor)
• Committee member: Lana Škrgatić (predsjednik povjerenstva)
• Committee member: Dinka Pavičić Baldani (član povjerenstva)
• Committee member: Ivana Karmelić (član povjerenstva)
• Committee member: Ksenija Fumić (član povjerenstva)
• Committee member: Dunja Rogić (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2024-07-03, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Clinical Biochemistry
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Gynecology and Obstetrics
• Universal decimal classification (UDC): 618 - Gynaecology. Obstetrics

Abstract

Staničnim metabolizmom ili djelovanjem egzogenih tvari nastaju slobodni radikali koji sudjeluju u međustaničnoj signalizaciji, a njihovo prekomjerno stvaranje oštećuje makromolekule. Cilj istraživanja bio je po prvi puta odrediti biljege lipidne peroksidacije u folikularnoj tekućini (FT) između modificiranog prirodnog ciklusa (mPC) i ciklusa s ovarijskom stimulacijom (OS) u ispitanica s idiopatskom neplodnosti. Također pokušala se ispitati razlika između razine biljega u FT 15-F2t-izoprostana (15-F2t-IsoP), malondialdehida (MDA), vitamina E i superoksid-dismutaze (SOD) s obzirom na godine, indeks tjelesne mase (BMI) i status pušenja te embriološke parametre i ishod liječenja. U istraživanje je uključeno 30 ispitanica u mPC i 30 ispitanica u OS ciklusu. Biljezi MDA, 15-F2t-IsoP, vitamin E i enzim SOD zadržavaju slične koncentracije tijekom godina. Povišena koncentracija MDA i vitamina E upućuje na prisutnost oksidacijskog stresa u skupini ispitanica visokog BMI dok status pušenja ne mijenja značajno biljege lipidne peroksidacije u FT. Prooksidacijski učinak u FT karakteriziran je statistički značajno višom koncentracijom MDA u ovarijskoj stimulaciji. Status zrelosti jajne stanice ne utječe na razine biljega redoks homeostaze. U FT-i uspješno fertiliziranih oocita postoji trend povišene koncentracije MDA koji nije statistički značajan. Isti trend zajedno s višom koncentracijom vitamina E prisutan je u suboptimalno ocijenjenim embrijima. Izraženija aktivnost SOD upućuje na prooksidacijsko okruženje jajnih stanica kod ispitanica s neostvarenim trudnoćama. Biljezi MDA, 15-F2t-IsoP, vitamin E i SOD ne mogu se koristiti u predviđanju ishoda u smislu uspješnosti fertilizacije, kvalitete embrija ili trudnoće. Ovaj doktorski rad daje doprinos sveobuhvatnijem uvidu u status redoks homeostaze u FT-i obzirom na pristup liječenja. Nastale promijene mogu kasnije utjecati na kvalitetu embrija i na ostvarenje trudnoće.

Abstract (english)

Reactive oxygen species are products of cell metabolism or can be generated by exogenous factors. A certain amount of ROS is needed for cell signalisation but excessive amount can cause cellular injury. This study aimed to determine for the first time redox homeostasis in follicular fluid (FF) between modified natural cycle (mNC) and ovarian stimulation (OS). Women with idiopathic infertility were recruited in the study. Additionally, a comparison was made between 15-F2t-isoprostane (15-F2t-IsoP), malondialdehyde (MDA), vitamin E and superoxide dismutase (SOD) regarding age, BMI, smoking status, embryological parameters and treatment outcome. The study included 30 women in mNC and 30 women in OC. Ageing and smoking status does not affect concentrations of MDA, 15-F2t-IsoP, vitamin E and activity of SOD. Although the differences were not statistically significant, oxidative stress is present in obesity through higher concentrations of MDA and vitamin E. Higher MDA in women with OS reflects prooxidant activity in FF. The state of oocyte maturity does not affect redox homeostasis. The trend to higher levels of MDA are present in FF from successfully fertilized oocyte but the differences are not statistically significant. The same trend with MDA and vitamin E levels is noticed in suboptimal embryos. Higher SOD activity reflects a prooxidant state in FF in the group with unsuccessful pregnancy. The levels of MDA, 15-F2t-IsoP, vitamin E and SOD can not predict successful fertilization, embryo quality and pregnancy success. This study contributes to a more comprehensive insight into the status of redox homeostasis regarding the choice of treatment. Changes can also affect embryo quality and pregnancy outcomes.

• Keywords: Trudnoća; Ishod trudnoće; 8-epi-prostaglandin F2alfa; Superoksid dismutaza; Izoprostani; Vitamin E; Folikularna tekućina; Malondialdehid; Indeks tjelesne mase; Oksidativni stres; Ishod liječenja; Starenje; Neplodnost; Pretilost; Gnojidba; Pušenje
• Keywords (english): Pregnancy; Pregnancy Outcome; 8-epi-prostaglandin F2alpha; Superoxide Dismutase; Isoprostanes; Vitamin E; Follicular Fluid; Malondialdehyde; Body Mass Index; Oxidative Stress; Treatment Outcome; Aging; Infertility; Obesity; Fertilization; Smoking
• Language: croatian
• URN:NBN: urn:nbn:hr:105:263361
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
• Type of resource: Text
• Extent: 91 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-07-23 08:33:06