prikaz prve stranice dokumenta Ceftriaxone Inhibits Conditioned Fear and Compulsive-like Repetitive Marble Digging without Central Nervous System Side Effects Typical of Diazepam—A Study on DBA2/J Mice and a High-5HT Subline of Wistar–Zagreb 5HT Rats
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Scientific paper - Original scientific paper
Ceftriaxone Inhibits Conditioned Fear and Compulsive-like Repetitive Marble Digging without Central Nervous System Side Effects Typical of Diazepam—A Study on DBA2/J Mice and a High-5HT Subline of Wistar–Zagreb 5HT Rats
Biomedicines, 12 (2024), 8; 1711. https://doi.org/10.3390/biomedicines12081711
Poljak, Ljiljana; Miše, Branko; Čičin-Šain, Lipa; Tvrdeić, Ante

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Poljak, Lj., Miše, B., Čičin Šain, L. & Tvrdeić, A. (2024). Ceftriaxone Inhibits Conditioned Fear and Compulsive-like Repetitive Marble Digging without Central Nervous System Side Effects Typical of Diazepam—A Study on DBA2/J Mice and a High-5HT Subline of Wistar–Zagreb 5HT Rats. Biomedicines, 12. (8). doi: 10.3390/biomedicines12081711

Poljak, Ljiljana, et al. "Ceftriaxone Inhibits Conditioned Fear and Compulsive-like Repetitive Marble Digging without Central Nervous System Side Effects Typical of Diazepam—A Study on DBA2/J Mice and a High-5HT Subline of Wistar–Zagreb 5HT Rats." Biomedicines, vol. 12, no. 8, 2024. https://doi.org/10.3390/biomedicines12081711

Poljak, Ljiljana, Branko Miše, Lipa Čičin Šain and Ante Tvrdeić. "Ceftriaxone Inhibits Conditioned Fear and Compulsive-like Repetitive Marble Digging without Central Nervous System Side Effects Typical of Diazepam—A Study on DBA2/J Mice and a High-5HT Subline of Wistar–Zagreb 5HT Rats." Biomedicines 12, no. 8 (2024). https://doi.org/10.3390/biomedicines12081711

Poljak, Lj., et al. (2024) 'Ceftriaxone Inhibits Conditioned Fear and Compulsive-like Repetitive Marble Digging without Central Nervous System Side Effects Typical of Diazepam—A Study on DBA2/J Mice and a High-5HT Subline of Wistar–Zagreb 5HT Rats', Biomedicines, 12(8). doi: 10.3390/biomedicines12081711

Poljak Lj, Miše B, Čičin Šain L, Tvrdeić A. Ceftriaxone Inhibits Conditioned Fear and Compulsive-like Repetitive Marble Digging without Central Nervous System Side Effects Typical of Diazepam—A Study on DBA2/J Mice and a High-5HT Subline of Wistar–Zagreb 5HT Rats. Biomedicines [Internet]. 2024 August 01 [cited 2025 April 04];12(8). doi: 10.3390/biomedicines12081711

Lj. Poljak, B. Miše, L. Čičin Šain and A. Tvrdeić, "Ceftriaxone Inhibits Conditioned Fear and Compulsive-like Repetitive Marble Digging without Central Nervous System Side Effects Typical of Diazepam—A Study on DBA2/J Mice and a High-5HT Subline of Wistar–Zagreb 5HT Rats", Biomedicines, vol. 12, no. 8, August 2024. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:022316. [Accessed: 04 April 2025]

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Title (english)Ceftriaxone Inhibits Conditioned Fear and Compulsive-like Repetitive Marble Digging without Central Nervous System Side Effects Typical of Diazepam—A Study on DBA2/J Mice and a High-5HT Subline of Wistar–Zagreb 5HT Rats
AuthorLjiljana Poljak
AuthorBranko Miše
AuthorLipa Čičin-Šain
AuthorAnte Tvrdeić
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Physiology and Immunology)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Pharmacology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Pharmacology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Physiology
Abstract (english)
Background: Ceftriaxone upregulates GLT1 glutamate transporter in the brain and may have anti-CFC and anti-OCD effects. Methods: Twenty WZ-5HT rats were used to investigate the effects of ceftriaxone on obsessive–compulsive (OCD)-like behaviour in the marble-burying (MB) test, freezing behaviour in contextual fear conditioning (CFC) and expression of GLT1 protein in the hippocampus or amygdala using immunoblots. Fifteen DBA/2J mice were used in the MB test. We also compared diazepam with ceftriaxone in open-field, beam-walking, and wire-hanging tests on 47 DBA/2J mice. Ceftriaxone (200 mg/kg) and saline were applied intraperitoneally, once daily for 7 (rats) or 5 (mice) consecutive days. A single dose of diazepam (1.5–3.0 mg/kg) or saline was injected 30 min before the behavioural tests. Results: Ceftriaxone significantly diminished OCD-like behaviour (↓ number of marbles buried) and freezing behaviour in CFC context session (↑ latencies, ↓ total duration, ↓ duration over four 2 min periods of the session) but increased GLT1 protein expression in the amygdala and hippocampus of rats. Diazepam induced sedation, ataxia and myorelaxation in mice. Ceftriaxone did not have these side effects. Conclusions: The results of this study confirm the anti-CFC and anti-OCD effects of ceftriaxone, which did not produce the unwanted effects typical of diazepam.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleBiomedicines
Numberingvol. 12, no. 8, 1711
e-ISSN2227-9059
DOIhttps://doi.org/10.3390/biomedicines12081711
URN:NBNurn:nbn:hr:105:022316
Publication2024-08-01
Document URLhttps://www.mdpi.com/2227-9059/12/8/1711
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2025-03-25 12:38:07