Izolacija i diferencijacija matičnih stanica iz sluznice usne šupljine

master's thesis

• Title (english): Isolation and differentiation of stem cells from oral mucosa
• Author: Filip Keršić
• Mentor: Dinko Mitrečić (mentor)
• Committee member: Marija Ćurlin (predsjednik povjerenstva)
• Committee member: Nataša Jovanov Milošević (član povjerenstva)
• Committee member: Dinko Mitrečić (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Histology and Embryology); Zagreb
• Defense date and country: 2016-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Cytology, Histology and Embryology

Abstract

Matične stanice sluznice usne šupljine su prvi put opisane prije pet godina. Kao i
druge matične stanice, posjeduju tri jedinstvene karakteristike koje ih razlikuju od bilo koje
druge stanice u našem tijelu: samoobnavljanje, klonalnost te sposobnost diferenciranja u
nekoliko staničnih vrsta. Budući da su matične stanice sluznice usne šupljine podrijetla
neuralnog grebena, pokazuju sklonost diferenciranja prema živčanoj staničnoj lozi. Također,
ove stanice pokazuju antibakterijska te imunomodulacijska svojstva. Zbog ovih karakteristika,
obećavaju puno u terapiji brojnih bolesti.
Cilj ovog istraživanja bio je napraviti in vitro analizu diferencijacije matičnih stanica
sluznice usne šupljine te dokazati njihovu sposobnost diferencijacije prema ektodermalnoj i
mezodermalnoj staničnoj lozi. Rezultati su pokazali da se te stanice doista mogu diferencirati
prema ektodermalnoj lozi pri čemu su izražavale biljege matičnih stanica (Oct4, Nestin i
SOX2) te neurona (MAP2 i β3-tubulin). Također, izražavajući biljeg Osterix smo dokazali i
njihovu sposobnost diferencijacije prema mezodermalnoj staničnoj lozi.

Abstract (english)

Human oral mucosa stem cells (hOMSC) are a novel, stem cell population that was
described five years ago. As other stem cells, they possess three unique properties that make
them different from any other cell in our body. Those are: self-renewal, clonality and potency
to generate several differentiated cell types. hOMSCs are multipotent and have the ability to
differentiate down mesenchymal and neuronal lineages. What is more, these cells demonstrate
antibacterial and immunomodulatory properties. Due to these characteristics, they hold great
promises for the treatment of many pathologies.
The goal of our study was to perform in vitro analysis of differentiation of stem
cells from oral mucosa and prove their capacity to differentiate down ectodermal and
mesodermal lineage. As a result, we proved that those cells indeed have capacity to
differentiate into ectodermal lineage by expressing stem cell markers (Oct4, Nestin and
SOX2), as well as neuronal markers (MAP2 and ß3-tubulin). Moreover, by expressing Osterix
marker we proved that those cells can differentiate into mesodermal lineage.

• Keywords: matične stanice sluznice usne šupljine; jedinstvene karakteristike; multipotentnost; imunomodulacija
• Keywords (english): human oral mucosa stem cells; unique properties; multipotency; immunomodulation
• Language: croatian
• URN:NBN: urn:nbn:hr:105:674234
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-08-18 08:22:31