Imunohistokemijska izraženost proteina BORIS i STAM-2 u tumorima zametnih stanica testisa

master's thesis

• Title (english): Immunohistochemical expression of BORIS and STAM-2 in testicular germ cell tumours
• Author: Ivana Milošević
• Mentor: Božo Krušlin (mentor)
• Committee member: Davor Tomas (predsjednik povjerenstva)
• Committee member: Slavko Gašparov (član povjerenstva)
• Committee member: Božo Krušlin (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Pathology); Zagreb
• Defense date and country: 2019-07-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Pathology

Abstract

Tumori zametnih stanica testisa su najčešći tumori mladih muškaraca u periodu od puberteta do 40 godina. U Republici Hrvatskoj i Republici Sloveniji se bilježi najveći porast incidencije TGCT-a od svih zemalja Europe. Naglasak ove studije je na skupini seminoma i neseminoma adolescenata i mlađe odrasle dobi. U ovom istraživanju po prvi put je analizirana imunohistokemijska izraženost BORIS-a i STAM-2 u TGCT-ima, na uzorku od 61 pacijenta. Ova dva proteina sudjeluju u unutarstaničnoj signalizaciji te se smatra da su povezani s aktivacijom protoonkogena. Utvrđena je pozitivna reakcija na BORIS u 100% analiziranih uzoraka, neovisno o histološkoj građi, dok je reakcija na STAM-2 bila slaba ili je nije bilo. Uočena je razlika u izraženosti STAM-2 u čistim seminomima i seminomskoj komponenti MGCT-a u odnosu na ostale komponente MGCT-a. Iako rezultati ovog istraživanja zahtijevaju daljnje potvrde, ukazuju na potencijalno značenje određivanja ekspresije BORIS-a i STAM-2 u TGCT-ima te njihovom eventualnom doprinosu poboljšanju dijagnostike ovih tumora.

Abstract (english)

Testicular germ cell tumours are the most common tumours in young males between puberty and the age of 40. Of all European countries the highest increase of incidence is registered in Republic of Croatia and Republic of Slovenia. The aim of this study was to analyse seminomatous and nonseminomatous tumours of adolescents and younger males. This study was the first to analyse the immunohistochemical expression of BORIS and STAM-2 in TGCTs, in 61 samples. These two proteins play a role in intracellular signalisation and are considered to be involved in activation of protooncogenes. All analysed samples were BORIS-positive, regardless of histological type, whereas the reaction to STAM-2 was weak or absent. There was a difference in the expression of STAM-2 in pure seminomatous tumours and seminomatous component of MGCTs in comparison with other components of MGCTs. Although this study requires further confirmation, it shows the potential significance of determining BORIS and STAM-2 expression and their possible contribution in the improvement of diagnosing TGCTs.

• Keywords: tumori zametnih stanica testisa; BORIS; STAM-2; imunohistokemija
• Keywords (english): testicular germ cell tumours; BORIS; STAM-2; immunohistochemistry
• Language: croatian
• URN:NBN: urn:nbn:hr:105:601804
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-12-12 09:42:19