Analiza infracrvenih spektara krvne plazme kao personaliziranog markera u dijagnostičko-terapijskom praćenju tumora dječje dobi

master's thesis

• Title (english): Analysis of blood plasma infrared spectrum as a personalized marker in diagnostic-therapeutic monitoring of pediatric cancer
• Author: Slavica Martinović
• Mentor: Ana Katušić (mentor)
• Committee member: Floriana Jakuš (predsjednik povjerenstva)
• Committee member: Ljiljana Šerman (član povjerenstva)
• Committee member: Ana Katušić (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Medical Biology); Zagreb
• Defense date and country: 2019-07-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Medical Microbiology

Abstract

Suvremena medicina danas stremi ka korištenju neinvazivnih kemijskih analiza tjelesnih tekućina kao što su krvna plazma, likvor, urin i druge. Traže se fine analitičke metode koje bi u idealnom slučaju uputile na dijagnozu i još važnije, prediktirale tijek bolesti u tjelesnim tekućinama. U ovom originalnom translacijskom istraživanju FTIR spektroskopijom su obrađeni arhivski uzorci krvne plazme pacijenata s dijagnozom pedijatrijske leukemije te u fazama terapije. Definirana je metoda snimanja i broj potrebnih spektara koji su potom analizirani statističkim metodama PCA i PC regresije. Spektri pacijenata uspoređeni sa spektrima arhivskih uzoraka plazme kontrolne skupine. Rezultati uvijek pokazuju jasno razdvajanje spektara pacijenata pri dijagnozi u usporedbi s spektrima kontrolne skupine. U kontroli, spektri svih pojedinaca grupiraju se u jednu skupinu. Spektri pacijenata međusobno su odvojeni što upućuje na različitost u stadiju prilikom postavljanja dijagnoze. Nadalje, analizom spektara svakog pacijenta zasebno uočen je statistički značajan obrazac protokola liječenja, koji se smanjuje pri puliranju spektara svih pacijenata. Rezultati upućuju na nužnost personaliziranog pristupa liječenju i naglašavaju važnost dubljeg translacijskog istraživanja FTIR spektroskopije i sličnih metoda u finom probiru zdravih i bolesnih te otkrivanju odgovora na terapiju.

Abstract (english)

Nowadays, a modern medicine tends to use non-invasive chemical analyses of body fluids such as blood plasma, liquor, urine, and others. For those, fine analytical methods are required which could ideally set up the diagnosis and, more importantly, detect the progress of the disease in body fluids. In this original translational study, by FTIR spectroscopy we have analyzed the archive blood plasma samples of patients with pediatric leukemia diagnosis and in the treatment phases. We had optimized the spectral recording method and defined the optimal number of spectra for every sample, and the spectra were then analyzed by statistical methods of PCA and PC regression. Patients’ spectra were compared with the spectra of archive plasma samples of the control group. The results always showed a clear separation of patient’s spectra in the time of diagnosis when compared to the control group. In controls, the spectra of all individuals were fitted into one group. The spectra of each patients were separated from others, which indicates the existing difference in the disease stage in the time of diagnosis. Furthermore, by analyzing the spectra of individual patient, a statistically significant predictable pattern of treatment protocol was visible separately, which was less visible when the spectra of all patients are fitted together. The results indicate the necessity of a personalized treatment approach and emphasize the importance of a deeper translational research of usage of the FTIR spectroscopy and similar methods in fine screening of healthy and sick persons and in the detection of the therapy response.

• Keywords: FTIR; plazma; leukemija; dječji tumori
• Keywords (english): FTIR; plasma; leukemia; pediatric cancer
• Language: croatian
• URN:NBN: urn:nbn:hr:105:865524
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-12-12 10:10:23