An automated mini buffy coat preparation method for use in mini extracorporeal photopheresis treatment of graft-vs-host-disease in a low body weight pediatric patient

Scientific paper - Original scientific paper

• Author: Toni Matić
• Author: Ines Bojanić
• Author: Sanja Mazić
• Author: Branka Golubić Cepulić
• Author: Ernest Bilić
• Author's institution: University of Zagreb; School of Medicine; (Department of Pediatrics)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Pediatrics

Abstract (english)

A mini extracorporeal photopheresis (mini-ECP) "off line" technique has been developed for use in the treatment of small children and patients with apheresis contraindications. Until now various methods have been used for buffy coat separation from whole blood. In this report we describe a protocol for mini buffy coat preparation using the automated Sepax laboratory separator for "off line" ECP treatment in a low body weight child with graft-vs-host-disease. According to our results this alternative method has been proven feasible and tolerable.

• Keywords (english): Blood Buffy Coat / cytology; Blood Component Removal / methods; Body Weight; Child; Child, Preschool; Female; Graft vs Host Disease / therapy; Humans; Male; Photopheresis / methods; Treatment Outcome
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Accepted version
• Journal title: Journal of Clinical Apheresis
• Numbering: vol. 34, no. 4, pp. 468-473
• p-ISSN: 0733-2459
• e-ISSN: 1098-1101
• DOI: https://doi.org/10.1002/jca.21700
• URN:NBN: urn:nbn:hr:105:587362
• Publication: 2019-08
• Document URL: http://doi.wiley.com/10.1002/jca.21700
• Type of resource: Text
• Access conditions: Open access
• Created on: 2021-02-01 11:31:57