Učinak pentadekapeptida BPC 157 na kolateralnu cirkulaciju nakon akutne okluzije abdominalne aorte štakora

doctoral thesis

• Title (english): Effect of pentadecapeptide BPC 157 on collateral circulation after acute occlusion of the abdominal aorta in rats
• Author: Katarina Kašnik Kovač https://orcid.org/0000-0001-8110-9849
• Mentor: Alenka Boban Blagaić (mentor)
• Mentor: Ivica Sjekavica (komentor)
• Committee member: Vinko Vidjak (predsjednik povjerenstva)
• Committee member: Andrija Hebrang (član povjerenstva)
• Committee member: Tomislav Meštrović (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Pathology); Zagreb
• Defense date and country: 2020-10-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Pharmacology
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Pathology
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Radiology
• Universal decimal classification (UDC): 616 - Pathology. Clinical medicine

Abstract

Istraženi učinak pentadekapeptida BPC 157 na kolateralnu cirkulaciju nakon akutne okluzije abdominalne aorte pokazao je da poveća protok kroz kolateralnu cirkulaciju od mjesta ligacije i djeluje protektivno na tkiva. Na animalnom modelu potpune ligature abdominalne aorte kirurškim šavom (distalno od polazišta desne iliolumbalne arterije, a proksimalno od bifurkacije abdominalne aorte) ispitan je učinak BPC 157 u tretmanu (10 μg/kg i.p.), u predtretmanu BPC 157 otopljen u vodi u dozi 10 μg/kg (0,25 μg/ml, 12 ml/štakor/dan, p.o. kroz 7 dana prije ligacije). Za kontrolne skupine korišten je ekvivalentni volumen 0,9 % NaCl (tretman) i voda za piće (predtretman). Istraživanje je provedeno na ženkama štakora soja Wistar, po 10 životinja u svakoj skupini (ukupno 160 životinja) na vremenskim intervalima od 1 min, 1 h, 6 h i 24 h. Analizirana je kolateralna cirkulacija pomoću digitalne subtrakcijske angiografije (DSA), dok je broj krvnih žila kolona i uterusa analiziran pomoću mikrokamere. Kod skupina na intervalima od 6 h i 24 h izvršena je analiza hodne pruge. Nakon žrtvovanja, uzeti su uzorci za patohistološku analizu. Pomoću DSA dokazana je statistički značajna (P < 0,05) veća srednja vrijednost broja kolaterala u svim BPC 157 (tretman i predtretman) skupinama. Direktnom opservacijom mikrokamerom dokazan je veći ukupan broj krvnih žila u području descendentnog kolona i uterusa kod svih BPC 157 skupina u odnosu na kontrolu. Rezultati su potvrđeni patohistološkom analizom navedenog tkiva. Analiza hodne pruge (6 h i 24 h) je pokazala značajno bolji hod kod BPC 157 skupina. BPC 157 je pokazao preventivni i terapijski učinak kod okluzije abdominalne aorte uz aktivaciju kolateralne cirkulacije, što predstavlja potencijal ovog peptida u terapiji okluzivnih bolesti.

Abstract (english)

In this study, it was demonstrated that BPC 157 increased the blood flow through the collateral circulation from the ligation site and acted protectively on the tissues. On animal model of the complete ligature with surgical suture of the abdominal aorta (distal from the starting point of the right iliolumbar artery and proximal to the bifurcation of the abdominal aorta), the examination was done on, the effect of BPC 157 (10 μg/kg i.p.) in treatment, in pre-treatment BPC 157 dissolved in a drinking water at a dose of 10 μg/kg p.o. (0.25 μg/ml, 12 ml/rat/day for 7 days prior to ligation). For control, an equivalent volume of 0.9% NaCl (treatment) and drinking water (pretreatment) was used. Tests were conducted on female Wistar rats, 10 animals in each group (altogether 160 animals) at time intervals of 1min, 1h, 6h, and 24h. Collateral circulation was analyzed using digital subtraction angiography (DSA), while the number of blood vessels in the colon and uterus were analyzed using a micro camera. The walking test was performed in groups at intervals of 6 h and 24 h. After sacrificing, samples were taken for pathohistological analysis. With DSA, there was shown a statistically significant (P < 0.05) higher mean value of collaterals in all BPC 157 (treatment and pre-treatment) groups. Using a micro camera, a higher total number of blood vessels in the descendent colon and uterus in BPC 157 groups in relation to controls was demonstrated. The results were confirmed by a pathohistological analysis of the tissues. The assessment of the walking test (6 h and 24 h) showed a significantly better course in all BPC 157 groups. BPC 157 showed a preventive and therapeutic effect in occlusion of the abdominal aorta with the activation of collateral circulation, which represents the potential of this peptide in the treatment of occlusive diseases.

• Keywords: pentadekapeptid BPC 157; okluzija abdominalne aorte; kolateralna cirkulacija
• Keywords (english): pentadecapeptide BPC 157; occlusion of the abdominal aorta; collateral circulation
• Language: croatian
• URN:NBN: urn:nbn:hr:105:933325
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
• Catalog URL: https://katalog.nsk.hr/F/19IT7Y755V1NXQJPHCLYSQGPF6BLAPPF179HQ7FAXXMYBYGMV5-21672?func=find-e&request=U%C4%8Dinak+pentadekapeptida+BPC+157+na+kolateralnu+cirkulaciju+nakon+akutne+okluzije+abdominalne+aorte+%C5%A1takora&find_scan_code=FIND_NAS&adjacent=N&local_base=MF_WEB&x=0&y=0&filter_code_1=WLN&filter_request_1=&filter_code_2=WYR&filter_request_2=&filter_code_3=WYR&filter_request_3=&filter_code_4=WFM&filter_request_4=
• Type of resource: Text
• Extent: 107 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-03-19 10:22:12