Biomarkeri razgradnje i stvaranja kolagena III, IV i V u serumu kao pokazatelji upalne aktivnosti u bolesnika s upalnim bolestima crijeva

doctoral thesis

• Title (english): Biomarkers of degradation and formation of collagen III, IV and V in serum as markers of inflammatory activity in patients with inflammatory bowel disease
• Author: Viktor Domislović; VIAF: 43160242715552430914
• Mentor: Željko Krznarić (mentor)
• Committee member: Silvija Čuković-Čavka (predsjednik povjerenstva)
• Committee member: Marko Banić (član povjerenstva)
• Committee member: Neven Ljubičić (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2021, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Internal Medicine
• Universal decimal classification (UDC): 616 - Pathology. Clinical medicine

Abstract

Ulcerozni kolitis (UC) i Crohnova bolest (CB) zahtijevaju kontinuiranu evaluaciju
aktivnosti bolesti. Izvanstanični matriks (ECM) se sastoji od bazalne membrane koja
se sastoji većinom od kolagena IV i intersticijskog matriksa u čijem se sastavu nalaze
kolageni I, III i V. Patološko okruženje dovodi do narušenog remodeliranja, strukture,
kvalitete i funkcije ECM-a. Naš cilj je bio istražiti biomarkere razgradnje i stvaranja
kolagena i njihovu povezanost s aktivnosti bolesti u ispitanika s upalnim bolestima
crijeva. U ovoj presječnoj studiji biomarkeri remodeliranja ECM-a izmjereni su u 162
ispitanika (110 CB i 52 UC). Biomarkeri razgradnje kolagena III (C3M) i stvaranja (Pro-
C3), razgradnje kolagena IV (C4M) i stvaranja (Pro-C4) i stvaranja kolagena V (Pro-
C5) izmjereni su metodom ELISA. Upalna aktivnost procijenjena je klinički, biokemijski
i endoskopski. U aktivnoj upali prevladavaju procesi razgradnje kolagena III i IV i
procesi stvaranja kolagena IV i V, dok u fazi remisije prevladava proces izgradnje
kolagena III. Postoji razlika u profilu biomarkera izvanstaničnog matriksa u upalnim
bolestima crijeva kada se upala definira koristeći endoskopske, kliničke ili biokemijske
pokazatelje upalne aktivnosti. Biomarkeri C3M, Pro-C3 i C4M i njihova kombinacija
mogli bi biti korisni u razlikovanju ispitanika s umjerenom do teškom aktivnosti bolesti
od ispitanika u remisiji.

Abstract (english)

Ulcerative colitis (UC) and Crohn's disease (CD) require continuous evaluation of
disease activity. Extracellular matrix (ECM) consists of basement membrane and
interstitial matrix, which consist mainly of type IV collagen and types I, III and V
collagen, respectively. Pathological environment leads to impaired remodeling,
structure, quality and function of ECM. Our aim was to investigate biomarkers of
collagen degradation and formation and their association with disease activity in
patients with IBD. In this cross-sectional study, we have measured five biomarkers of
ECM remodelling in 162 IBD patients (110 CD and 52 UC). Biomarkers of type III
collagen degradation (C3M) and formation (Pro-C3), type IV collagen degradation
(C4M) and formation (Pro-C4), and type V collagen formation (Pro-C5) were
measured in serum by ELISA. Inflammatory activity was assesed using clinical,
biochemical and endoscopic scores. We report pronounced process of collagen type
III and IV degradation and collagen type IV and V formation in active disease, and
formation of type III collagen in remission. There is a difference in ECM remodelling
profile in IBD using clinical, biochemical and endoscopic activity indices. Combinatinon
of biomarkers C3M, Pro-C3 and C4M could be useful in diferentiation patients with
moderate to severe active disease from patients in remission.

• Keywords: izvanstanični matriks; kolagen; upala; upalne bolesti crijeva
• Keywords (english): extracelullar matrix; collagen; inflammation; inflammatory bowel disease
• Language: croatian
• URN:NBN: urn:nbn:hr:105:673015
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
• Type of resource: Text
• Extent: 83 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-03-24 13:14:48