Učinak pentadekapeptida BPC 157 na cijeljenje vezikovaginalne fistule u štakora

doctoral thesis

• Title (english): Effect of pentadecapeptide BPC 157 on healing of vesicovaginal fistula in rats
• Author: Domagoj Rašić; VIAF: 305666753
• Mentor: Alenka Boban Blagaić (mentor)
• Mentor: Marko Sever (komentor)
• Committee member: Krešimir Bulić (predsjednik povjerenstva)
• Committee member: Željko Kaštelan (član povjerenstva)
• Committee member: Damir Babić (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2021-04-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Pathology
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Pharmacology
• Universal decimal classification (UDC): 615 - Pharmacology. Therapeutics. Toxicology ; 616 - Pathology. Clinical medicine

Abstract

Uvod: Vezikovaginalna fistula je patološki spoj između mokraćnog mjehura i vagine, predstavlja velike poteškoće u liječenju. Pojavljuje se kao komplikacija kirurških zahvata, porođaja i zračenja. Zahtijeva dugotrajno liječenje s brojnim kirurškim zahvatima. ----- Hipoteza: Pentadekapeptid BPC 157 dan parenteralno ili oralno može poboljšati cijeljenje vezikovaginalne fistule štakora. ----- Cilj: Pretpostavljamo pozitivan učinak pentadekapeptida BPC 157 u terapiji vezikovaginalnih fistula u štakora. ----- Materijali i metode: Za model vezikovaginalne fistule korištene su ženke Wistar štakora. Učinjena je incizija na prednjem zidu vagine i stražnjem zidu mokraćnog mjehura duljine 4 mm i formirana je vezikovaginalna fistula. Pentadekapaptid BPC 157 primijenjen je u dnevnim dozama od 1 μg/kg, 10 μg/kg, 100 ng/kg, 10 ng/kg i.p. tijekom cijelog trajanja eksperimenta, počevši 14 dana nakon operacije. Kontrolnoj skupini životinja je dana dnevna ekvivalentna količina 0,9% NaCl i.p. Nakon žrtvovanja promatran je i uspoređivan promjer fistule, makroskopske promjene i razlike u zarastanju fistula među skupinama te je uzeto tkivo za daljnju patohistološku analizu. ----- Rezultati: Fistula je zacijelila kod svih štakora tretiranih BPC 157 te je isti doveo do porasta volumena potrebnog za istjecanje tekućine kroz fistulu. Zaključak: Pentadekapeptid BPC 157 poboljšao je zarastanje vezikovaginalne fistule.

Abstract (english)

Introduction: Vesicovaginal fistula is anomalous connection between bladder and vagina, represents great difficulty in healing. It occurs as a complication of surgical procedures, childbirth and radiation. It requires long-term treatment often with number of surgical procedures. -----
Hypothesis: Pentadecapeptide BPC 157 administered parenterally or orally can improve the healing of vesicovaginal fistula in rats. -----
Aims: We hypothesize positive effect of the pentadecapeptide BPC 157 in therapy of vesicovaginal fistulas in rats.
Materials and methods: For the model of vesicovaginal fistula female Wistar rats were used. An incision was made on anterior vaginal wall and rear urinary blader wall in length of 4 mm and vesicovaginal fistula was formed. Pentadecapaptide BPC 157 was applied in daily doses of 1 μg/kg, 10 μg/kg, 100 ng/kg, 10 ng/kg i.p. during the experiment, starting 14 days after surgery and control group of animals was given daily equivalent amount of 0,9% NaCl i.p. After sacrifice, diameter of fistulas, macroscopic changes and differences in fistula healing between groups were observed and compared. Tissue was taken for microscopic analysis. -----
Results: Fistula was successfully healed in all rats that underwent BPC 157 therapy. Also, volume needed to leakage of fluid through fistula was increased. -----
Conclusion: Pentadecapeptide BPC 157 improved vesicovaginal fistula healing.

• Keywords: pentadekapeptid BPC 157; vezikovaginalna fistula; štakori
• Keywords (english): pentadecapeptide BPC 157; vesicovaginal fistula; rats
• Language: croatian
• URN:NBN: urn:nbn:hr:105:245378
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
• Type of resource: Text
• Extent: 115 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-05-05 11:53:12