prikaz prve stranice dokumenta Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays
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Scientific paper - Original scientific paper
Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays
Scientific Reports, 11 (2021), 1; 6001. https://doi.org/10.1038/s41598-021-85438-5
Wolff, Noa; Hendling, Michaela; Schroeder, Fabian; Schönthaler, Silvia; Geiss, Andreas F.; Bedenić, Branka; Barišić, Ivan

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Wolff, N., Hendling, M., Schroeder, F., Schönthaler, S., Geiss, A. F., Bedenić, B. & Barišić, I. (2021). Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays. Scientific Reports, 11. (1). doi: 10.1038/s41598-021-85438-5

Wolff, Noa, et al. "Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays." Scientific Reports, vol. 11, no. 1, 2021. https://doi.org/10.1038/s41598-021-85438-5

Wolff, Noa, Michaela Hendling, Fabian Schroeder, Silvia Schönthaler, Andreas F. Geiss, Branka Bedenić and Ivan Barišić. "Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays." Scientific Reports 11, no. 1 (2021). https://doi.org/10.1038/s41598-021-85438-5

Wolff, N., et al. (2021) 'Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays', Scientific Reports, 11(1). doi: 10.1038/s41598-021-85438-5

Wolff N, Hendling M, Schroeder F, Schönthaler S, Geiss AF, Bedenić B, and sur.. Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays. Scientific Reports [Internet]. 2021 March 16 [cited 2024 April 12];11(1). doi: 10.1038/s41598-021-85438-5

N. Wolff, et al., "Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays", Scientific Reports, vol. 11, no. 1, March 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:422074. [Accessed: 12 April 2024]

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Metadata
Title (english)Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays
AuthorNoa Wolff
AuthorMichaela Hendling
AuthorFabian Schroeder
AuthorSilvia Schönthaler
AuthorAndreas F. Geiss
AuthorBranka Bedenić
AuthorIvan Barišić
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
Antibiotic resistances progressively cause treatment failures, and their spreading dynamics reached an alarming level. Some strains have already been classified as highly critical, e.g. the ones summarised by the acronym ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp.). To restrain this trend and enable effective medication, as much information as possible must be obtained in the least possible time. Here, we present a DNA microarray-based assay that screens for the most important sepsis-relevant 44 pathogenic species, 360 virulence factors (mediate pathogenicity in otherwise non-pathogenic strains), and 409 antibiotic resistance genes in parallel. The assay was evaluated with 14 multidrug resistant strains, including all ESKAPE pathogens, mainly obtained from clinical isolates. We used a cost-efficient ligation-based detection platform designed to emulate the highly specific multiplex detection of padlock probes. Results could be obtained within one day, requiring approximately 4 h for amplification, application to the microarray, and detection.
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleScientific Reports
Numberingvol. 11, no. 1, 6001
e-ISSN2045-2322
DOIhttps://doi.org/10.1038/s41598-021-85438-5
URN:NBNurn:nbn:hr:105:422074
Publication2021-03-16
Document URLhttp://www.nature.com/articles/s41598-021-85438-5
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2021-06-18 08:05:59