Kalretinin u djagnostici Hirschsprungove bolesti

master's thesis

• Title (english): Calretinin in diagnosis of Hirschsprung's disease
• Author: Antonia Čivljak
• Mentor: Ruža Grizelj (mentor)
• Committee member: Jurica Vuković (predsjednik povjerenstva)
• Committee member: Marija Jelušić (član povjerenstva)
• Committee member: Ruža Grizelj (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Pediatrics); Zagreb
• Defense date and country: 2021-07-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Pediatrics

Abstract

UVOD: Istraživanje je provedeno s ciljem ispitivanja i usporedbe dijagnostičke vrijednosti standardnih metoda histološke analize (bojenje hemalaun-eozinom, histokemijsko bojenje acetilkolinesteraze) s imunohistokemijskim bojenjem na kalretinin bioptata rektuma dobivenih sukcijskom biopsijom u bolesnika sa sumnjom na Hirschsprungovu bolest. ----- METODE: Provedena je retrospektivna analiza medicinske dokumentacije sve djece kojima je od siječnja 2016. do svibnja 2021. godine u KBC-u Zagreb rađena sukcijska biopsija rektuma zbog sumnje na Hirschsprungovu bolest. Izdvojena su osnovna obilježja bolesnika i nalazi dijagnostičkih i terapijskih postupaka. Za usporedbu dijagnostičkih testova izračunata je specifičnost, osjetljivost, pozitivna prediktivna vrijednost i negativna prediktivna vrijednost. ----- REZULTATI: Analizirani su podatci 24 ispitanika. Dijagnoza Hirschsprungove bolesti potvrđena je u sedam (29%) bolesnika. Najčešći razlog sumnje na Hirschsprungovu bolest i indikacija za izvođenje sukcijske biopsije rektuma bili su distenzija abdomena (66,7%), opstipacija (62,5%) i povraćanje (41,7%). U postavljanju dijagnoze Hirschsprungove bolesti najveću specifičnost (85%) i osjetljivost (100%) pokazalo je imunohistokemijsko bojenje na kalretinin, a najmanju specifičnost (66,7%) i osjetljivost (37,5%) histokemijsko bojenje acetilkolinesteraze. ----- ZAKLJUČAK: Imunohistokemijsko bojenje na kalretinin ima visoku dijagnostičku točnost. Uvođenje imunohistokemijskog bojenja na kalretinin u standardni dijagnostički postupak moglo bi ubrzati i unaprijediti točnost dijagnostičkog postupka otklanjanjem poteškoća koje nerijetko otežavaju pravovremeno postavljanje dijagnoze Hirschsprungove bolesti.

Abstract (english)

INTRODUCTION: The study was conducted to examine and compare the diagnostic values of standard methods of histological analysis (hematoxylin and eosin staining, histochemical staining for acetylcholinesterase) with immunohistochemical staining for calretinin of rectal biopsies obtained by rectal suction biopsy in patients with suspected Hirschsprung’s disease. ----- METHODS: A retrospective analysis of the medical records of all children who underwent a rectal suction biopsy from January 2016 to May 2021 at the University Hospital Center Zagreb due to suspected Hirschsprung's disease. The basic characteristics of the patient and the findings of diagnostic and therapeutic procedures were extracted. Specificity, sensitivity, positive predictive value, and negative predictive value were calculated to compare diagnostic tests. ----- RESULTS: Data from 24 participants were analyzed. The diagnosis of Hirschsprung's disease was confirmed in seven (29%) patients. The most common reasons for suspicion of Hirschsprung's disease and indications for performing a rectal suction biopsy were abdominal distension (66.7%), constipation (62.5%) and vomiting (41.7%). In the diagnosis of Hirschsprung's disease, immunohistochemical staining for calretinin showed the highest specificity (85%) and sensitivity (100%), and histochemical staining of acetylcholinesterase showed the lowest specificity (66.7%) and sensitivity (37.5%). ----- CONCLUSION: Immunohistochemical staining of calretinin has high diagnostic accuracy. The introduction of immunohistochemical staining for calretinin in the standard diagnostic procedure could accelerate and improve the accuracy of the diagnostic procedure by eliminating difficulties that often complicate the timely diagnosis of Hirschsprung's disease.

• Keywords: Hirschsprungova bolest; sukcijska biopsija rektuma; imunohistokemijsko bojenje na kalretinin; bojenje hemalaun-eozinom; histokemijsko bojenje acetilkolinesteraze
• Keywords (english): Hirschsprung's disease; suction rectal biopsy; immunohistochemical staining for calretinin; hematoxylin and eosin staining; histochemical staining for acetylcholinesterase
• Language: croatian
• URN:NBN: urn:nbn:hr:105:608072
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-01-28 13:30:29