Ganglioside Composition Distinguishes Anaplastic Ganglioglioma Tumor Tissue from Peritumoral Brain Tissue: Complementary Mass Spectrometry and Thin-Layer Chromatography Evidence

Scientific paper - Case study

• Author: Dragana Fabris
• Author: Ivana Karmelić
• Author: Hasan Muharemović
• Author: Tomislav Sajko
• Author: Mia Jurilj
• Author: Slavica Potočki
• Author: Ruđer Novak
• Author: Željka Vukelić
• Author's institution: University of Zagreb; School of Medicine; (Department of Medical Chemistry, Biochemistry and Clinical Chemistry)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Medical Biochemistry

Abstract (english)

Gangliosides serve as antitumor therapy targets and aberrations in their composition strongly correlate with tumor growth and invasiveness. Anaplastic ganglioglioma is a rare, poorly characterized, malignant neuronal–glial tumor type. We present the first comparative characterization of ganglioside composition in anaplastic ganglioglioma vs. peritumoral and healthy brain tissues by combining mass spectrometry and thin-layer chromatography. Anaplastic ganglioglioma ganglioside composition was highly distinguishable from both peritumoral and healthy tissue despite having five to six times lower total content. Ten out of twelve MS-identified ganglioside classes, defined by unique glycan residues, were represented by a large number and considerable abundance of individual species with different fatty acid residues (C16–C24) in ceramide portions. The major structurally identified class was tumor-associated GD3 (>50%) with 11 species; GD3 (d18:1/24:0) being the most abundant. The dominant sphingoid base residue in ganglioside ceramides was sphingosine (d18:1), followed by eicosasphingosine (d20:1). The peritumoral tissue ganglioside composition was estimated as normal. Specific ganglioside composition and large variability of ganglioside ceramide structures determined in anaplastic ganglioglioma demonstrate realistic ganglioside expression patterns and correspond to the profile of high-grade malignancy brain tumors.

• Keywords (english): Aged; Brain / metabolism; Brain / pathology; Brain Neoplasms / metabolism; Brain Neoplasms / pathology; Carcinoma / metabolism; Carcinoma / pathology; Chromatography, Thin Layer / methods; Female; Ganglioglioma / metabolism; Ganglioglioma / pathology; Gangliosides / analysis; Gangliosides / metabolism; Humans; Mass Spectrometry / methods; Middle Aged
• Language: english
• Publication type: Scientific paper - Case study
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: International Journal of Molecular Sciences
• Numbering: vol. 22, no. 16, 8844
• e-ISSN: 1422-0067
• DOI: https://doi.org/10.3390/ijms22168844
• URN:NBN: urn:nbn:hr:105:528352
• Publication: 2021-08-17
• Document URL: https://www.mdpi.com/1422-0067/22/16/8844
• Type of resource: Text
• Access conditions: Open access
• Created on: 2022-05-12 07:30:35