Comprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based X-ray phase contrast imaging

Scientific paper - Original scientific paper

• Author: Ivo Planinc
• Author: Patricia Garcia-Canadilla
• Author: Hector Dejea
• Author: Ivana Ilic
• Author: Eduard Guasch
• Author: Monica Zamora
• Author: Fàtima Crispi
• Author: Marco Stampanoni
• Author: Davor Milicic
• Author: Bart Bijnens
• Author: Anne Bonnin
• Author: Maja Čikes
• Author's institution: University of Zagreb; School of Medicine; (Department of Internal Medicine)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Internal Medicine

Abstract (english)

Abstract Cardiovascular research is in an ongoing quest for a superior imaging method to integrate gross-anatomical information with microanatomy, combined with quantifiable parameters of cardiac structure. In recent years, synchrotron radiation-based X-ray Phase Contrast Imaging (X-PCI) has been extensively used to characterize soft tissue in detail. The objective was to use X-PCI to comprehensively quantify ischemic remodeling of different myocardial structures, from cell to organ level, in a rat model of myocardial infarction. Myocardial infarction-induced remodeling was recreated in a well-established rodent model. Ex vivo rodent hearts were imaged by propagation based X-PCI using two configurations resulting in 5.8 µm and 0.65 µm effective pixel size images. The acquired datasets were used for a comprehensive assessment of macrostructural changes including the whole heart and vascular tree morphology, and quantification of left ventricular myocardial thickness, mass, volume, and organization. On the meso-scale, tissue characteristics were explored and compared with histopathological methods, while microstructural changes were quantified by segmentation of cardiomyocytes and calculation of cross-sectional areas. Propagation based X-PCI provides detailed visualization and quantification of morphological changes on whole organ, tissue, vascular as well as individual cellular level of the ex vivo heart, with a single, non-destructive 3D imaging modality.

• Keywords (english): Animals; Coronary Vessels / diagnostic imaging; Diagnostic Imaging / methods; Disease Models, Animal; Image Processing, Computer-Assisted; Imaging, Three-Dimensional; Male; Microscopy, Phase-Contrast; Myocardial Ischemia / diagnostic imaging; Myocardial Ischemia / pathology; Myocardial Ischemia / therapy; Percutaneous Coronary Intervention; Rats; Synchrotrons; Ventricular Remodeling; X-Rays
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: Scientific Reports
• Numbering: vol. 11, no. 1, 14020
• e-ISSN: 2045-2322
• DOI: https://doi.org/10.1038/s41598-021-93054-6
• URN:NBN: urn:nbn:hr:105:371839
• Publication: 2021-07-07
• Document URL: http://www.nature.com/articles/s41598-021-93054-6
• Type of resource: Text
• Access conditions: Open access
• Created on: 2022-06-09 09:26:38